Rice auxin influx carrier OsAUX1 facilitates root hair elongation in response to low external phosphate

Jitender Giri; Rahul Bhosale; Guoqiang Huang; Bipin K. Pandey; Helen Parker; Susan Zappala; Jing Yang; Anne Dievart; Charlotte Bureau; Karin Ljung; Adam Price; Terry Rose; Antoine Larrieu; Stefan Mairhofer; Craig J. Sturrock; Philip White; Lionel Dupuy; Malcolm Hawkesford; Christophe Perin; Wanqi Liang; Benjamin Peret; Charlie T. Hodgman; Jonathan Lynch; Matthias Wissuwa; Dabing Zhang; Tony Pridmore; Sacha J. Mooney; Emmanuel Guiderdoni; Ranjan Swarup; Malcolm J. Bennett

doi:10.1038/s41467-018-03850-4

Rice auxin influx carrier OsAUX1 facilitates root hair elongation in response to low external phosphate

Jitender Giri, Rahul Bhosale, Guoqiang Huang, Bipin K. Pandey, Helen Parker, Susan Zappala, Jing Yang, Anne Dievart, Charlotte Bureau, Karin Ljung, Adam Price, Terry Rose, Antoine Larrieu, Stefan Mairhofer, Craig J. Sturrock, Philip White, Lionel Dupuy, Malcolm Hawkesford, Christophe Perin, Wanqi LiangBenjamin Peret, Charlie T. Hodgman, Jonathan Lynch, Matthias Wissuwa, Dabing Zhang, Tony Pridmore, Sacha J. Mooney, Emmanuel Guiderdoni, Ranjan Swarup, Malcolm J. Bennett

Research output: Contribution to journal › Article › peer-review

108 Scopus citations

Abstract

Root traits such as root angle and hair length influence resource acquisition particularly for immobile nutrients like phosphorus (P). Here, we attempted to modify root angle in rice by disrupting the OsAUX1 auxin influx transporter gene in an effort to improve rice P acquisition efficiency. We show by X-ray microCT imaging that root angle is altered in the osaux1 mutant, causing preferential foraging in the top soil where P normally accumulates, yet surprisingly, P acquisition efficiency does not improve. Through closer investigation, we reveal that OsAUX1 also promotes root hair elongation in response to P limitation. Reporter studies reveal that auxin response increases in the root hair zone in low P environments. We demonstrate that OsAUX1 functions to mobilize auxin from the root apex to the differentiation zone where this signal promotes hair elongation when roots encounter low external P. We conclude that auxin and OsAUX1 play key roles in promoting root foraging for P in rice.

Original language	English (US)
Article number	1408
Journal	Nature communications
Volume	9
Issue number	1
DOIs	https://doi.org/10.1038/s41467-018-03850-4
State	Published - Dec 1 2018

All Science Journal Classification (ASJC) codes

Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
Physics and Astronomy(all)

Access to Document

10.1038/s41467-018-03850-4

Cite this

Giri, J., Bhosale, R., Huang, G., Pandey, B. K., Parker, H., Zappala, S., Yang, J., Dievart, A., Bureau, C., Ljung, K., Price, A., Rose, T., Larrieu, A., Mairhofer, S., Sturrock, C. J., White, P., Dupuy, L., Hawkesford, M., Perin, C., ... Bennett, M. J. (2018). Rice auxin influx carrier OsAUX1 facilitates root hair elongation in response to low external phosphate. Nature communications, 9(1), Article 1408. https://doi.org/10.1038/s41467-018-03850-4

@article{9137bd3136974a91ab8bb015390faa8d,

title = "Rice auxin influx carrier OsAUX1 facilitates root hair elongation in response to low external phosphate",

abstract = "Root traits such as root angle and hair length influence resource acquisition particularly for immobile nutrients like phosphorus (P). Here, we attempted to modify root angle in rice by disrupting the OsAUX1 auxin influx transporter gene in an effort to improve rice P acquisition efficiency. We show by X-ray microCT imaging that root angle is altered in the osaux1 mutant, causing preferential foraging in the top soil where P normally accumulates, yet surprisingly, P acquisition efficiency does not improve. Through closer investigation, we reveal that OsAUX1 also promotes root hair elongation in response to P limitation. Reporter studies reveal that auxin response increases in the root hair zone in low P environments. We demonstrate that OsAUX1 functions to mobilize auxin from the root apex to the differentiation zone where this signal promotes hair elongation when roots encounter low external P. We conclude that auxin and OsAUX1 play key roles in promoting root foraging for P in rice.",

author = "Jitender Giri and Rahul Bhosale and Guoqiang Huang and Pandey, {Bipin K.} and Helen Parker and Susan Zappala and Jing Yang and Anne Dievart and Charlotte Bureau and Karin Ljung and Adam Price and Terry Rose and Antoine Larrieu and Stefan Mairhofer and Sturrock, {Craig J.} and Philip White and Lionel Dupuy and Malcolm Hawkesford and Christophe Perin and Wanqi Liang and Benjamin Peret and Hodgman, {Charlie T.} and Jonathan Lynch and Matthias Wissuwa and Dabing Zhang and Tony Pridmore and Mooney, {Sacha J.} and Emmanuel Guiderdoni and Ranjan Swarup and Bennett, {Malcolm J.}",

note = "Funding Information: This work was supported by the awards from the Biotechnology and Biological Sciences Research Council [grant numbers BB/G023972/1, BB/R013748/1, BB/L026848/1, BB/M018431/1, BB/PO16855/1, BB/M001806/1, BB/P010520/1]; the European Research Council FUTUREROOTS Advanced Investigator grant [grant number 294729]; Leverhulme Trust [grant number RPG-2016-409]; Royal Society [grant number WM130021, NA140281]; Newton International Fellowship (NF140287) and British Council Newton Bhabha (228144076). This work was also supported by funds from the University of Nottingham Future Food Beacon of Excellence Nottingham Research and PhD+ fellowship schemes; the Interuniversity Attraction Poles Program initiated by the Belgian Science Policy Office [P7/29]; the Swedish Governmental Agency for Innovation Systems (VINNOVA), and the Swedish Research Council (V.R.) to K.L. We also thank Roger Granbom (Swedish University of Agricultural Sciences) for technical assistance and Gabriel Castrillo for commenting on the manuscript text. Part of this work has been conducted at the Rice Functional Genomics REFUGE platform funded by Agropolis Fondation in Montpellier, France. Publisher Copyright: {\textcopyright} 2018 The Author(s).",

year = "2018",

month = dec,

day = "1",

doi = "10.1038/s41467-018-03850-4",

language = "English (US)",

volume = "9",

journal = "Nature communications",

issn = "2041-1723",

publisher = "Nature Publishing Group",

number = "1",

}

Giri, J, Bhosale, R, Huang, G, Pandey, BK, Parker, H, Zappala, S, Yang, J, Dievart, A, Bureau, C, Ljung, K, Price, A, Rose, T, Larrieu, A, Mairhofer, S, Sturrock, CJ, White, P, Dupuy, L, Hawkesford, M, Perin, C, Liang, W, Peret, B, Hodgman, CT, Lynch, J, Wissuwa, M, Zhang, D, Pridmore, T, Mooney, SJ, Guiderdoni, E, Swarup, R & Bennett, MJ 2018, 'Rice auxin influx carrier OsAUX1 facilitates root hair elongation in response to low external phosphate', Nature communications, vol. 9, no. 1, 1408. https://doi.org/10.1038/s41467-018-03850-4

TY - JOUR

T1 - Rice auxin influx carrier OsAUX1 facilitates root hair elongation in response to low external phosphate

AU - Giri, Jitender

AU - Bhosale, Rahul

AU - Huang, Guoqiang

AU - Pandey, Bipin K.

AU - Parker, Helen

AU - Zappala, Susan

AU - Yang, Jing

AU - Dievart, Anne

AU - Bureau, Charlotte

AU - Ljung, Karin

AU - Price, Adam

AU - Rose, Terry

AU - Larrieu, Antoine

AU - Mairhofer, Stefan

AU - Sturrock, Craig J.

AU - White, Philip

AU - Dupuy, Lionel

AU - Hawkesford, Malcolm

AU - Perin, Christophe

AU - Liang, Wanqi

AU - Peret, Benjamin

AU - Hodgman, Charlie T.

AU - Lynch, Jonathan

AU - Wissuwa, Matthias

AU - Zhang, Dabing

AU - Pridmore, Tony

AU - Mooney, Sacha J.

AU - Guiderdoni, Emmanuel

AU - Swarup, Ranjan

AU - Bennett, Malcolm J.

N1 - Funding Information: This work was supported by the awards from the Biotechnology and Biological Sciences Research Council [grant numbers BB/G023972/1, BB/R013748/1, BB/L026848/1, BB/M018431/1, BB/PO16855/1, BB/M001806/1, BB/P010520/1]; the European Research Council FUTUREROOTS Advanced Investigator grant [grant number 294729]; Leverhulme Trust [grant number RPG-2016-409]; Royal Society [grant number WM130021, NA140281]; Newton International Fellowship (NF140287) and British Council Newton Bhabha (228144076). This work was also supported by funds from the University of Nottingham Future Food Beacon of Excellence Nottingham Research and PhD+ fellowship schemes; the Interuniversity Attraction Poles Program initiated by the Belgian Science Policy Office [P7/29]; the Swedish Governmental Agency for Innovation Systems (VINNOVA), and the Swedish Research Council (V.R.) to K.L. We also thank Roger Granbom (Swedish University of Agricultural Sciences) for technical assistance and Gabriel Castrillo for commenting on the manuscript text. Part of this work has been conducted at the Rice Functional Genomics REFUGE platform funded by Agropolis Fondation in Montpellier, France. Publisher Copyright: © 2018 The Author(s).

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Root traits such as root angle and hair length influence resource acquisition particularly for immobile nutrients like phosphorus (P). Here, we attempted to modify root angle in rice by disrupting the OsAUX1 auxin influx transporter gene in an effort to improve rice P acquisition efficiency. We show by X-ray microCT imaging that root angle is altered in the osaux1 mutant, causing preferential foraging in the top soil where P normally accumulates, yet surprisingly, P acquisition efficiency does not improve. Through closer investigation, we reveal that OsAUX1 also promotes root hair elongation in response to P limitation. Reporter studies reveal that auxin response increases in the root hair zone in low P environments. We demonstrate that OsAUX1 functions to mobilize auxin from the root apex to the differentiation zone where this signal promotes hair elongation when roots encounter low external P. We conclude that auxin and OsAUX1 play key roles in promoting root foraging for P in rice.

AB - Root traits such as root angle and hair length influence resource acquisition particularly for immobile nutrients like phosphorus (P). Here, we attempted to modify root angle in rice by disrupting the OsAUX1 auxin influx transporter gene in an effort to improve rice P acquisition efficiency. We show by X-ray microCT imaging that root angle is altered in the osaux1 mutant, causing preferential foraging in the top soil where P normally accumulates, yet surprisingly, P acquisition efficiency does not improve. Through closer investigation, we reveal that OsAUX1 also promotes root hair elongation in response to P limitation. Reporter studies reveal that auxin response increases in the root hair zone in low P environments. We demonstrate that OsAUX1 functions to mobilize auxin from the root apex to the differentiation zone where this signal promotes hair elongation when roots encounter low external P. We conclude that auxin and OsAUX1 play key roles in promoting root foraging for P in rice.

UR - http://www.scopus.com/inward/record.url?scp=85045513892&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85045513892&partnerID=8YFLogxK

U2 - 10.1038/s41467-018-03850-4

DO - 10.1038/s41467-018-03850-4

M3 - Article

C2 - 29650967

AN - SCOPUS:85045513892

SN - 2041-1723

VL - 9

JO - Nature communications

JF - Nature communications

IS - 1

M1 - 1408

ER -

Rice auxin influx carrier OsAUX1 facilitates root hair elongation in response to low external phosphate

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this