Rice auxin influx carrier OsAUX1 facilitates root hair elongation in response to low external phosphate

Jitender Giri; Rahul Bhosale; Guoqiang Huang; Bipin K. Pandey; Helen Parker; Susan Zappala; Jing Yang; Anne Dievart; Charlotte Bureau; Karin Ljung; Adam Price; Terry Rose; Antoine Larrieu; Stefan Mairhofer; Craig J. Sturrock; Philip White; Lionel Dupuy; Malcolm Hawkesford; Christophe Perin; Wanqi Liang; Benjamin Peret; Charlie T. Hodgman; Jonathan Lynch; Matthias Wissuwa; Dabing Zhang; Tony Pridmore; Sacha J. Mooney; Emmanuel Guiderdoni; Ranjan Swarup; Malcolm J. Bennett

doi:10.1038/s41467-018-03850-4

Rice auxin influx carrier OsAUX1 facilitates root hair elongation in response to low external phosphate

Jitender Giri, Rahul Bhosale, Guoqiang Huang, Bipin K. Pandey, Helen Parker, Susan Zappala, Jing Yang, Anne Dievart, Charlotte Bureau, Karin Ljung, Adam Price, Terry Rose, Antoine Larrieu, Stefan Mairhofer, Craig J. Sturrock, Philip White, Lionel Dupuy, Malcolm Hawkesford, Christophe Perin, Wanqi LiangBenjamin Peret, Charlie T. Hodgman, Jonathan Lynch, Matthias Wissuwa, Dabing Zhang, Tony Pridmore, Sacha J. Mooney, Emmanuel Guiderdoni, Ranjan Swarup, Malcolm J. Bennett

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Abstract

Root traits such as root angle and hair length influence resource acquisition particularly for immobile nutrients like phosphorus (P). Here, we attempted to modify root angle in rice by disrupting the OsAUX1 auxin influx transporter gene in an effort to improve rice P acquisition efficiency. We show by X-ray microCT imaging that root angle is altered in the osaux1 mutant, causing preferential foraging in the top soil where P normally accumulates, yet surprisingly, P acquisition efficiency does not improve. Through closer investigation, we reveal that OsAUX1 also promotes root hair elongation in response to P limitation. Reporter studies reveal that auxin response increases in the root hair zone in low P environments. We demonstrate that OsAUX1 functions to mobilize auxin from the root apex to the differentiation zone where this signal promotes hair elongation when roots encounter low external P. We conclude that auxin and OsAUX1 play key roles in promoting root foraging for P in rice.

Original language	English (US)
Article number	1408
Journal	Nature communications
Volume	9
Issue number	1
DOIs	https://doi.org/10.1038/s41467-018-03850-4
State	Published - Dec 1 2018

All Science Journal Classification (ASJC) codes

General Chemistry
General Biochemistry, Genetics and Molecular Biology
General Physics and Astronomy

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Giri, J., Bhosale, R., Huang, G., Pandey, B. K., Parker, H., Zappala, S., Yang, J., Dievart, A., Bureau, C., Ljung, K., Price, A., Rose, T., Larrieu, A., Mairhofer, S., Sturrock, C. J., White, P., Dupuy, L., Hawkesford, M., Perin, C., ... Bennett, M. J. (2018). Rice auxin influx carrier OsAUX1 facilitates root hair elongation in response to low external phosphate. Nature communications, 9(1), Article 1408. https://doi.org/10.1038/s41467-018-03850-4

@article{9137bd3136974a91ab8bb015390faa8d,

title = "Rice auxin influx carrier OsAUX1 facilitates root hair elongation in response to low external phosphate",

abstract = "Root traits such as root angle and hair length influence resource acquisition particularly for immobile nutrients like phosphorus (P). Here, we attempted to modify root angle in rice by disrupting the OsAUX1 auxin influx transporter gene in an effort to improve rice P acquisition efficiency. We show by X-ray microCT imaging that root angle is altered in the osaux1 mutant, causing preferential foraging in the top soil where P normally accumulates, yet surprisingly, P acquisition efficiency does not improve. Through closer investigation, we reveal that OsAUX1 also promotes root hair elongation in response to P limitation. Reporter studies reveal that auxin response increases in the root hair zone in low P environments. We demonstrate that OsAUX1 functions to mobilize auxin from the root apex to the differentiation zone where this signal promotes hair elongation when roots encounter low external P. We conclude that auxin and OsAUX1 play key roles in promoting root foraging for P in rice.",

author = "Jitender Giri and Rahul Bhosale and Guoqiang Huang and Pandey, {Bipin K.} and Helen Parker and Susan Zappala and Jing Yang and Anne Dievart and Charlotte Bureau and Karin Ljung and Adam Price and Terry Rose and Antoine Larrieu and Stefan Mairhofer and Sturrock, {Craig J.} and Philip White and Lionel Dupuy and Malcolm Hawkesford and Christophe Perin and Wanqi Liang and Benjamin Peret and Hodgman, {Charlie T.} and Jonathan Lynch and Matthias Wissuwa and Dabing Zhang and Tony Pridmore and Mooney, {Sacha J.} and Emmanuel Guiderdoni and Ranjan Swarup and Bennett, {Malcolm J.}",

note = "Publisher Copyright: {\textcopyright} 2018 The Author(s).",

year = "2018",

month = dec,

day = "1",

doi = "10.1038/s41467-018-03850-4",

language = "English (US)",

volume = "9",

journal = "Nature communications",

issn = "2041-1723",

publisher = "Nature Research",

number = "1",

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Giri, J, Bhosale, R, Huang, G, Pandey, BK, Parker, H, Zappala, S, Yang, J, Dievart, A, Bureau, C, Ljung, K, Price, A, Rose, T, Larrieu, A, Mairhofer, S, Sturrock, CJ, White, P, Dupuy, L, Hawkesford, M, Perin, C, Liang, W, Peret, B, Hodgman, CT, Lynch, J, Wissuwa, M, Zhang, D, Pridmore, T, Mooney, SJ, Guiderdoni, E, Swarup, R & Bennett, MJ 2018, 'Rice auxin influx carrier OsAUX1 facilitates root hair elongation in response to low external phosphate', Nature communications, vol. 9, no. 1, 1408. https://doi.org/10.1038/s41467-018-03850-4

TY - JOUR

T1 - Rice auxin influx carrier OsAUX1 facilitates root hair elongation in response to low external phosphate

AU - Giri, Jitender

AU - Bhosale, Rahul

AU - Huang, Guoqiang

AU - Pandey, Bipin K.

AU - Parker, Helen

AU - Zappala, Susan

AU - Yang, Jing

AU - Dievart, Anne

AU - Bureau, Charlotte

AU - Ljung, Karin

AU - Price, Adam

AU - Rose, Terry

AU - Larrieu, Antoine

AU - Mairhofer, Stefan

AU - Sturrock, Craig J.

AU - White, Philip

AU - Dupuy, Lionel

AU - Hawkesford, Malcolm

AU - Perin, Christophe

AU - Liang, Wanqi

AU - Peret, Benjamin

AU - Hodgman, Charlie T.

AU - Lynch, Jonathan

AU - Wissuwa, Matthias

AU - Zhang, Dabing

AU - Pridmore, Tony

AU - Mooney, Sacha J.

AU - Guiderdoni, Emmanuel

AU - Swarup, Ranjan

AU - Bennett, Malcolm J.

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Root traits such as root angle and hair length influence resource acquisition particularly for immobile nutrients like phosphorus (P). Here, we attempted to modify root angle in rice by disrupting the OsAUX1 auxin influx transporter gene in an effort to improve rice P acquisition efficiency. We show by X-ray microCT imaging that root angle is altered in the osaux1 mutant, causing preferential foraging in the top soil where P normally accumulates, yet surprisingly, P acquisition efficiency does not improve. Through closer investigation, we reveal that OsAUX1 also promotes root hair elongation in response to P limitation. Reporter studies reveal that auxin response increases in the root hair zone in low P environments. We demonstrate that OsAUX1 functions to mobilize auxin from the root apex to the differentiation zone where this signal promotes hair elongation when roots encounter low external P. We conclude that auxin and OsAUX1 play key roles in promoting root foraging for P in rice.

AB - Root traits such as root angle and hair length influence resource acquisition particularly for immobile nutrients like phosphorus (P). Here, we attempted to modify root angle in rice by disrupting the OsAUX1 auxin influx transporter gene in an effort to improve rice P acquisition efficiency. We show by X-ray microCT imaging that root angle is altered in the osaux1 mutant, causing preferential foraging in the top soil where P normally accumulates, yet surprisingly, P acquisition efficiency does not improve. Through closer investigation, we reveal that OsAUX1 also promotes root hair elongation in response to P limitation. Reporter studies reveal that auxin response increases in the root hair zone in low P environments. We demonstrate that OsAUX1 functions to mobilize auxin from the root apex to the differentiation zone where this signal promotes hair elongation when roots encounter low external P. We conclude that auxin and OsAUX1 play key roles in promoting root foraging for P in rice.

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U2 - 10.1038/s41467-018-03850-4

DO - 10.1038/s41467-018-03850-4

M3 - Article

C2 - 29650967

AN - SCOPUS:85045513892

SN - 2041-1723

VL - 9

JO - Nature communications

JF - Nature communications

IS - 1

M1 - 1408

ER -

Rice auxin influx carrier OsAUX1 facilitates root hair elongation in response to low external phosphate

Abstract

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