TY - JOUR
T1 - Rice chloroplast DNA molecules are heterogeneous as revealed by DNA sequences of a cluster of genes
AU - Moon, Eunpyo
AU - Kao, Teh Hui
AU - Wu, Ray
N1 - Funding Information:
ACKNOWLEDGEMENTS We thank Chris Howe for the wheat atpB and atpE clones, and Tom Fox and Margaret McCann for helpful comments. This work was supported by research grants RF84066, Allocation No. 3 from the Rockefeller Foundation, and GM29179 from the National Institutes of Health, U.S. Public Health Service.
PY - 1987/1/26
Y1 - 1987/1/26
N2 - We describe the isolation of two rice chloroplast HindIII fragments (9.5 kb and 5.3 kb) each containing a gene cluster coding for the large subunit of ribulose-l,5-bisphosphate carboxylase (rbcL), β and ε subunits of ATPase (atpB and atpE), tRNAmet (trnM) and tRNAval (trnV). All five genes contained in the 9.5 kb fragment are potentially functional, whereas in the 5.3 kb fragment, rbcL is truncated and atpB is frame-shift mutated. The copy number of the 9.5 kb fragment is 10 times that of the 5.3 kb fragment, indicating that the two fragments are probably located on different chloroplast genomes and represent two different (major and minor) genomic populations. Thus, the rice chloroplast genome appears to be heterogeneous, contrary to general belief. We also describe the isolation of a rice mitochondrial HindIII fragment (6.9 kb) which contains an almost complete transferred copy of this chloroplast gene cluster. In this transferred copy, the coding sequences of rbcL, atpE and trnM contain perfectly normal reading frames, whereas atpB has become grossly defective and trnV is truncated.
AB - We describe the isolation of two rice chloroplast HindIII fragments (9.5 kb and 5.3 kb) each containing a gene cluster coding for the large subunit of ribulose-l,5-bisphosphate carboxylase (rbcL), β and ε subunits of ATPase (atpB and atpE), tRNAmet (trnM) and tRNAval (trnV). All five genes contained in the 9.5 kb fragment are potentially functional, whereas in the 5.3 kb fragment, rbcL is truncated and atpB is frame-shift mutated. The copy number of the 9.5 kb fragment is 10 times that of the 5.3 kb fragment, indicating that the two fragments are probably located on different chloroplast genomes and represent two different (major and minor) genomic populations. Thus, the rice chloroplast genome appears to be heterogeneous, contrary to general belief. We also describe the isolation of a rice mitochondrial HindIII fragment (6.9 kb) which contains an almost complete transferred copy of this chloroplast gene cluster. In this transferred copy, the coding sequences of rbcL, atpE and trnM contain perfectly normal reading frames, whereas atpB has become grossly defective and trnV is truncated.
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U2 - 10.1093/nar/15.2.611
DO - 10.1093/nar/15.2.611
M3 - Article
C2 - 3029686
AN - SCOPUS:0023665426
SN - 0305-1048
VL - 15
SP - 611
EP - 630
JO - Nucleic acids research
JF - Nucleic acids research
IS - 2
ER -