RNA and beta;-hemolysin of group B streptococcus induce interleukin-1β (IL-1β) by activating NLRP3 inflammasomes in mouse macrophages

Rahul Gupta, Shubhendu Ghosh, Brian Monks, Rosane B. DeOliveira, Te Chen Tzeng, Parisa Kalantari, Anubhab Nandy, Bornali Bhattacharjee, Jennie Chan, Fabianno Ferreira, Vijay Rathinam, Shruti Sharma, Egil Lien, Neal Silverman, Katherine Fitzgerald, Arnaud Firon, Patrick Trieu-Cuot, Philipp Henneke, Douglas T. Golenbock

Research output: Contribution to journalArticlepeer-review

63 Scopus citations

Abstract

The inflammatory cytokine IL-1β is critical for host responses against many human pathogens. Here, we define Group B Strep-tococcus (GBS)-mediated activation of the Nod-like receptor-P3 (NLRP3) inflammasome in macrophages. NLRP3 activation requires GBS expression of the cytolytic toxin, β-hemolysin, lysosomal acidification, and leakage. These processes allow the interaction of GBS RNA with cytosolic NLRP3. The present study supports a model in which GBS RNA, along with lysosomal components including cathepsins, leaks out of lysosomes and interacts with NLRP3 to induce IL-1β production.

Original languageEnglish (US)
Pages (from-to)13701-13705
Number of pages5
JournalJournal of Biological Chemistry
Volume289
Issue number20
DOIs
StatePublished - May 16 2014

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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