RNA and beta;-hemolysin of group B streptococcus induce interleukin-1β (IL-1β) by activating NLRP3 inflammasomes in mouse macrophages

Rahul Gupta; Shubhendu Ghosh; Brian Monks; Rosane B. DeOliveira; Te Chen Tzeng; Parisa Kalantari; Anubhab Nandy; Bornali Bhattacharjee; Jennie Chan; Fabianno Ferreira; Vijay Rathinam; Shruti Sharma; Egil Lien; Neal Silverman; Katherine Fitzgerald; Arnaud Firon; Patrick Trieu-Cuot; Philipp Henneke; Douglas T. Golenbock

doi:10.1074/jbc.C114.548982

RNA and beta;-hemolysin of group B streptococcus induce interleukin-1β (IL-1β) by activating NLRP3 inflammasomes in mouse macrophages

Rahul Gupta, Shubhendu Ghosh, Brian Monks, Rosane B. DeOliveira, Te Chen Tzeng, Parisa Kalantari, Anubhab Nandy, Bornali Bhattacharjee, Jennie Chan, Fabianno Ferreira, Vijay Rathinam, Shruti Sharma, Egil Lien, Neal Silverman, Katherine Fitzgerald, Arnaud Firon, Patrick Trieu-Cuot, Philipp Henneke, Douglas T. Golenbock

Veterinary and Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

65 Scopus citations

Abstract

The inflammatory cytokine IL-1β is critical for host responses against many human pathogens. Here, we define Group B Strep-tococcus (GBS)-mediated activation of the Nod-like receptor-P3 (NLRP3) inflammasome in macrophages. NLRP3 activation requires GBS expression of the cytolytic toxin, β-hemolysin, lysosomal acidification, and leakage. These processes allow the interaction of GBS RNA with cytosolic NLRP3. The present study supports a model in which GBS RNA, along with lysosomal components including cathepsins, leaks out of lysosomes and interacts with NLRP3 to induce IL-1β production.

Original language	English (US)
Pages (from-to)	13701-13705
Number of pages	5
Journal	Journal of Biological Chemistry
Volume	289
Issue number	20
DOIs	https://doi.org/10.1074/jbc.C114.548982
State	Published - May 16 2014

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1074/jbc.C114.548982

Cite this

Gupta, R., Ghosh, S., Monks, B., DeOliveira, R. B., Tzeng, T. C., Kalantari, P., Nandy, A., Bhattacharjee, B., Chan, J., Ferreira, F., Rathinam, V., Sharma, S., Lien, E., Silverman, N., Fitzgerald, K., Firon, A., Trieu-Cuot, P., Henneke, P., & Golenbock, D. T. (2014). RNA and beta;-hemolysin of group B streptococcus induce interleukin-1β (IL-1β) by activating NLRP3 inflammasomes in mouse macrophages. Journal of Biological Chemistry, 289(20), 13701-13705. https://doi.org/10.1074/jbc.C114.548982

@article{d3cbe10251824cbc832f06febdfa476c,

title = "RNA and beta;-hemolysin of group B streptococcus induce interleukin-1β (IL-1β) by activating NLRP3 inflammasomes in mouse macrophages",

abstract = "The inflammatory cytokine IL-1β is critical for host responses against many human pathogens. Here, we define Group B Strep-tococcus (GBS)-mediated activation of the Nod-like receptor-P3 (NLRP3) inflammasome in macrophages. NLRP3 activation requires GBS expression of the cytolytic toxin, β-hemolysin, lysosomal acidification, and leakage. These processes allow the interaction of GBS RNA with cytosolic NLRP3. The present study supports a model in which GBS RNA, along with lysosomal components including cathepsins, leaks out of lysosomes and interacts with NLRP3 to induce IL-1β production.",

author = "Rahul Gupta and Shubhendu Ghosh and Brian Monks and DeOliveira, {Rosane B.} and Tzeng, {Te Chen} and Parisa Kalantari and Anubhab Nandy and Bornali Bhattacharjee and Jennie Chan and Fabianno Ferreira and Vijay Rathinam and Shruti Sharma and Egil Lien and Neal Silverman and Katherine Fitzgerald and Arnaud Firon and Patrick Trieu-Cuot and Philipp Henneke and Golenbock, {Douglas T.}",

year = "2014",

month = may,

day = "16",

doi = "10.1074/jbc.C114.548982",

language = "English (US)",

volume = "289",

pages = "13701--13705",

journal = "Journal of Biological Chemistry",

issn = "0021-9258",

publisher = "American Society for Biochemistry and Molecular Biology Inc.",

number = "20",

}

Gupta, R, Ghosh, S, Monks, B, DeOliveira, RB, Tzeng, TC, Kalantari, P, Nandy, A, Bhattacharjee, B, Chan, J, Ferreira, F, Rathinam, V, Sharma, S, Lien, E, Silverman, N, Fitzgerald, K, Firon, A, Trieu-Cuot, P, Henneke, P & Golenbock, DT 2014, 'RNA and beta;-hemolysin of group B streptococcus induce interleukin-1β (IL-1β) by activating NLRP3 inflammasomes in mouse macrophages', Journal of Biological Chemistry, vol. 289, no. 20, pp. 13701-13705. https://doi.org/10.1074/jbc.C114.548982

TY - JOUR

T1 - RNA and beta;-hemolysin of group B streptococcus induce interleukin-1β (IL-1β) by activating NLRP3 inflammasomes in mouse macrophages

AU - Gupta, Rahul

AU - Ghosh, Shubhendu

AU - Monks, Brian

AU - DeOliveira, Rosane B.

AU - Tzeng, Te Chen

AU - Kalantari, Parisa

AU - Nandy, Anubhab

AU - Bhattacharjee, Bornali

AU - Chan, Jennie

AU - Ferreira, Fabianno

AU - Rathinam, Vijay

AU - Sharma, Shruti

AU - Lien, Egil

AU - Silverman, Neal

AU - Fitzgerald, Katherine

AU - Firon, Arnaud

AU - Trieu-Cuot, Patrick

AU - Henneke, Philipp

AU - Golenbock, Douglas T.

PY - 2014/5/16

Y1 - 2014/5/16

N2 - The inflammatory cytokine IL-1β is critical for host responses against many human pathogens. Here, we define Group B Strep-tococcus (GBS)-mediated activation of the Nod-like receptor-P3 (NLRP3) inflammasome in macrophages. NLRP3 activation requires GBS expression of the cytolytic toxin, β-hemolysin, lysosomal acidification, and leakage. These processes allow the interaction of GBS RNA with cytosolic NLRP3. The present study supports a model in which GBS RNA, along with lysosomal components including cathepsins, leaks out of lysosomes and interacts with NLRP3 to induce IL-1β production.

AB - The inflammatory cytokine IL-1β is critical for host responses against many human pathogens. Here, we define Group B Strep-tococcus (GBS)-mediated activation of the Nod-like receptor-P3 (NLRP3) inflammasome in macrophages. NLRP3 activation requires GBS expression of the cytolytic toxin, β-hemolysin, lysosomal acidification, and leakage. These processes allow the interaction of GBS RNA with cytosolic NLRP3. The present study supports a model in which GBS RNA, along with lysosomal components including cathepsins, leaks out of lysosomes and interacts with NLRP3 to induce IL-1β production.

UR - http://www.scopus.com/inward/record.url?scp=84901020681&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84901020681&partnerID=8YFLogxK

U2 - 10.1074/jbc.C114.548982

DO - 10.1074/jbc.C114.548982

M3 - Article

C2 - 24692555

AN - SCOPUS:84901020681

SN - 0021-9258

VL - 289

SP - 13701

EP - 13705

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

IS - 20

ER -

RNA and beta;-hemolysin of group B streptococcus induce interleukin-1β (IL-1β) by activating NLRP3 inflammasomes in mouse macrophages

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this