RNA interference as a method for target-site screening in the western corn rootworm, Diabrotica virgifera virgifera

Analiza P. Alves, Marc D. Lorenzen, Richard W. Beeman, John E. Foster, Blair D. Siegfried

Research output: Contribution to journalArticlepeer-review

50 Scopus citations

Abstract

To test the efficacy of RNA interference (RNAi) as a method for target-site screening in Diabrotica virgifera virgifera LeConte (Coleptera: Chrysomelidae) larvae, genes were identified and tested for which clear RNAi phenotypes had been identified in the Coleopteran model, Tribolium castaneum. Here the cloning of the D. v. vergifera orthologs of laccase 2 (DvvLac2) and chitin synthase 2 (DvvCHS2) is reported. Injection of DvvLac2-specific double-stranded RNA resulted in prevention of post-molt cuticular tanning, while injection of DvvCHS2-specific dsRNA reduced chitin levels in midguts. Silencing of both DvvLac2 and DvvCHS2 was confirmed by RT-PCR and quantitative RT-PCR. As in T. castaneum, RNAi-mediated gene silencing is systemic in Diabrotica. The results indicate that RNAi-induced silencing of D. v. vergifera genes provides a powerful tool for identifying potential insecticide targets.

Original languageEnglish (US)
Article number162
JournalJournal of Insect Science
Volume10
DOIs
StatePublished - 2010

All Science Journal Classification (ASJC) codes

  • Insect Science

Fingerprint

Dive into the research topics of 'RNA interference as a method for target-site screening in the western corn rootworm, Diabrotica virgifera virgifera'. Together they form a unique fingerprint.

Cite this