TY - JOUR
T1 - RNA isolation from recalcitrant plant tissue
AU - Schultz, David J.
AU - Craig, Richard
AU - Cox-Foster, Diana L.
AU - Mumma, Ralph O.
AU - Medford, June I.
PY - 1994/12
Y1 - 1994/12
N2 - The isolation of high-quality RNA from various tissues (leaves, pedicels, glandular trichomes) of garden geranium (Pelargonium xhortorum) using various published methods is difficult due to numerous oxidizing compounds. A new RNA extraction method was developed through the combination and modification of two separate procedures (Rochester et al., 1986; Manning 1991). In addition to geranium tissues, this method is successful when used with other recalcitrant tissues such as mature needles of white pine (Pinus strobus) and mature leaves of poinsettia (Euphorbia pulcherrima). RNA quality was judged by spectrophotometric readings, denaturing agarose gels, and successful reverse transcription.
AB - The isolation of high-quality RNA from various tissues (leaves, pedicels, glandular trichomes) of garden geranium (Pelargonium xhortorum) using various published methods is difficult due to numerous oxidizing compounds. A new RNA extraction method was developed through the combination and modification of two separate procedures (Rochester et al., 1986; Manning 1991). In addition to geranium tissues, this method is successful when used with other recalcitrant tissues such as mature needles of white pine (Pinus strobus) and mature leaves of poinsettia (Euphorbia pulcherrima). RNA quality was judged by spectrophotometric readings, denaturing agarose gels, and successful reverse transcription.
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U2 - 10.1007/BF02669273
DO - 10.1007/BF02669273
M3 - Article
AN - SCOPUS:51249165228
SN - 0735-9640
VL - 12
SP - 310
EP - 316
JO - Plant Molecular Biology Reporter
JF - Plant Molecular Biology Reporter
IS - 4
ER -