Calcium alginate immobilization of Hyoscyamus muticus plant tissue culture is shown to enhance the rapid formation of sesquiterpenes in response to exposure to extracts of the fungal pathogen Rhizoctonia solani. The studies were carried out with suspension-cultured cells and roots at low tissue loadings to demonstrate that the enhancement is not due to either the degree of differentiation or the presence of metabolic gradients. The fungally elicited response of root tissue is shown to be much less sensitive to both the physical and chemical environment than free cell suspensions. By examining the effects of the immobilization components (Ca2+, alginate), and immobilization in barium alginate gels, it is concluded that the enhanced sesquiterpene production in immobilized tissue is mediated through calcium.
All Science Journal Classification (ASJC) codes
- Applied Microbiology and Biotechnology