Role of calcium and differentiation in enhanced sesquiterpene elicitation from calcium alginate-immobilized plant tissue

Wayne R. Curtis; Ping Wang; Arthur Humphrey

doi:10.1016/0141-0229(94)00061-U

Role of calcium and differentiation in enhanced sesquiterpene elicitation from calcium alginate-immobilized plant tissue

Wayne R. Curtis, Ping Wang, Arthur Humphrey

Chemical Engineering

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15 Scopus citations

Abstract

Calcium alginate immobilization of Hyoscyamus muticus plant tissue culture is shown to enhance the rapid formation of sesquiterpenes in response to exposure to extracts of the fungal pathogen Rhizoctonia solani. The studies were carried out with suspension-cultured cells and roots at low tissue loadings to demonstrate that the enhancement is not due to either the degree of differentiation or the presence of metabolic gradients. The fungally elicited response of root tissue is shown to be much less sensitive to both the physical and chemical environment than free cell suspensions. By examining the effects of the immobilization components (Ca²⁺, alginate), and immobilization in barium alginate gels, it is concluded that the enhanced sesquiterpene production in immobilized tissue is mediated through calcium.

Original language	English (US)
Pages (from-to)	554-557
Number of pages	4
Journal	Enzyme and Microbial Technology
Volume	17
Issue number	6
DOIs	https://doi.org/10.1016/0141-0229(94)00061-U
State	Published - Jun 1995

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Biochemistry
Applied Microbiology and Biotechnology

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10.1016/0141-0229(94)00061-U

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title = "Role of calcium and differentiation in enhanced sesquiterpene elicitation from calcium alginate-immobilized plant tissue",

abstract = "Calcium alginate immobilization of Hyoscyamus muticus plant tissue culture is shown to enhance the rapid formation of sesquiterpenes in response to exposure to extracts of the fungal pathogen Rhizoctonia solani. The studies were carried out with suspension-cultured cells and roots at low tissue loadings to demonstrate that the enhancement is not due to either the degree of differentiation or the presence of metabolic gradients. The fungally elicited response of root tissue is shown to be much less sensitive to both the physical and chemical environment than free cell suspensions. By examining the effects of the immobilization components (Ca2+, alginate), and immobilization in barium alginate gels, it is concluded that the enhanced sesquiterpene production in immobilized tissue is mediated through calcium.",

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AU - Curtis, Wayne R.

AU - Wang, Ping

AU - Humphrey, Arthur

PY - 1995/6

Y1 - 1995/6

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AB - Calcium alginate immobilization of Hyoscyamus muticus plant tissue culture is shown to enhance the rapid formation of sesquiterpenes in response to exposure to extracts of the fungal pathogen Rhizoctonia solani. The studies were carried out with suspension-cultured cells and roots at low tissue loadings to demonstrate that the enhancement is not due to either the degree of differentiation or the presence of metabolic gradients. The fungally elicited response of root tissue is shown to be much less sensitive to both the physical and chemical environment than free cell suspensions. By examining the effects of the immobilization components (Ca2+, alginate), and immobilization in barium alginate gels, it is concluded that the enhanced sesquiterpene production in immobilized tissue is mediated through calcium.

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Role of calcium and differentiation in enhanced sesquiterpene elicitation from calcium alginate-immobilized plant tissue

Abstract

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