TY - JOUR
T1 - Role of GAC63 in transcriptional activation mediated by the aryl hydrocarbon receptor
AU - Chen, Yong Heng
AU - Beischlag, Timothy V.
AU - Jeong, Hoon Kim
AU - Perdew, Gary H.
AU - Stallcup, Michael R.
PY - 2006/5/5
Y1 - 2006/5/5
N2 - The aryl hydrocarbon receptor (AHR), amember of the basic helix-loop-helix/Per-Arnt-Sim (bHLH-PAS) gene family, binds a variety of polycyclic aromatic hydrocarbons and mediates their toxic effects. GAC63 has been shown to act as a coactivator in nuclear receptor-mediated gene transcription. In this report, we demonstrate that GAC63 interacts with AHR through its bHLH-PAS domain. Overexpression of GAC63 greatly enhanced AHR-regulated reporter gene activity in a ligand-dependent manner in transient transfection assays. Upon ligand treatment, endogenous GAC63 was recruited to the xenobiotic response element of the mouse CYP1A1 gene, an AHR-responsive gene. Reduction of the endogenous GAC63 level by small interfering RNA inhibited transcriptional activation by AHR. These findings reveal a new function of GAC63 in AHR-mediated gene transcription.
AB - The aryl hydrocarbon receptor (AHR), amember of the basic helix-loop-helix/Per-Arnt-Sim (bHLH-PAS) gene family, binds a variety of polycyclic aromatic hydrocarbons and mediates their toxic effects. GAC63 has been shown to act as a coactivator in nuclear receptor-mediated gene transcription. In this report, we demonstrate that GAC63 interacts with AHR through its bHLH-PAS domain. Overexpression of GAC63 greatly enhanced AHR-regulated reporter gene activity in a ligand-dependent manner in transient transfection assays. Upon ligand treatment, endogenous GAC63 was recruited to the xenobiotic response element of the mouse CYP1A1 gene, an AHR-responsive gene. Reduction of the endogenous GAC63 level by small interfering RNA inhibited transcriptional activation by AHR. These findings reveal a new function of GAC63 in AHR-mediated gene transcription.
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U2 - 10.1074/jbc.M512537200
DO - 10.1074/jbc.M512537200
M3 - Article
C2 - 16513642
AN - SCOPUS:33744948050
SN - 0021-9258
VL - 281
SP - 12242
EP - 12247
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 18
ER -