TY - JOUR
T1 - Role of sonic hedgehog in branchiomotor neuron induction in zebrafish
AU - Chandrasekhar, Anand
AU - Warren, James T.
AU - Takahashi, Kana
AU - Schauerte, Heike E.
AU - Van Eeden, Fredericus J.M.
AU - Haffter, Pascal
AU - Kuwada, John Y.
N1 - Funding Information:
We thank Stephen C. Ekker, Mary Halloran, Wataru Shoji, and David Turner for critically reading the manuscript, Jim Lauderdale for discussion, Stephen C. Ekker and David Turner for expression plasmids, Bill Trevarrow for the zn5 antibody, Bonnie Ullman and Chuck Kimmel for cyclops fish, and Su Fengyun for fish care. A.C. is grateful to Christiane Nüsslein-Volhard for encouragement and support, and for providing laboratory facilities to conduct some of the experiments described here. The 39.4D5 islet and the 3A10 antibodies developed by Tom Jessell and Jane Dodd were obtained from the Developmental Studies Hybridoma Bank maintained by the Department of Biological Sciences, University of Iowa, under contract N01-HD-7-3263 from the NICHD. This work was supported by a Human Frontiers Science Programme Short-term Fellowship (A.C), a Paralyzed Veterans of America/Spinal Cord Research Foundation Fellowship (J.T.W.), and grants from the Muscular Dystrophy Association and NINDS NS24848 (J.Y.K).
PY - 1998/8
Y1 - 1998/8
N2 - The role of zebrafish hedgehog genes in branchiomotor neuron development was analyzed by examining mutations that affect the expression of the hedgehog genes and by overexpressing these genes in embryos. In cyclops mutants, reduction in sonic hedgehog (shh) expression, and elimination of tiggy-winkle hedgehog (twhh) expression, correlated with reductions in branchiomotor neuron populations. Furthermore, branchiomotor neurons were restored in cyclops mutants when shh or twhh was overexpressed. These results suggest that Shh and/or Twhh play an important role in the induction of branchiomotor neurons in vivo. In sonic-you (syu) mutants, where Shh activity was reduced or eliminated due to mutations in shh, branchiomotor neurons were reduced in number in a rhombomere-specific fashion, but never eliminated. Similarly, spinal motor neurons were reduced, but not eliminated, in syu mutants. These results demonstrate that Shh is not solely responsible for inducing branchiomotor and spinal motor neurons, and suggest that Shh and Twhh may function as partially redundant signals for motor neuron induction in zebrafish.
AB - The role of zebrafish hedgehog genes in branchiomotor neuron development was analyzed by examining mutations that affect the expression of the hedgehog genes and by overexpressing these genes in embryos. In cyclops mutants, reduction in sonic hedgehog (shh) expression, and elimination of tiggy-winkle hedgehog (twhh) expression, correlated with reductions in branchiomotor neuron populations. Furthermore, branchiomotor neurons were restored in cyclops mutants when shh or twhh was overexpressed. These results suggest that Shh and/or Twhh play an important role in the induction of branchiomotor neurons in vivo. In sonic-you (syu) mutants, where Shh activity was reduced or eliminated due to mutations in shh, branchiomotor neurons were reduced in number in a rhombomere-specific fashion, but never eliminated. Similarly, spinal motor neurons were reduced, but not eliminated, in syu mutants. These results demonstrate that Shh is not solely responsible for inducing branchiomotor and spinal motor neurons, and suggest that Shh and Twhh may function as partially redundant signals for motor neuron induction in zebrafish.
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U2 - 10.1016/S0925-4773(98)00101-4
DO - 10.1016/S0925-4773(98)00101-4
M3 - Article
C2 - 9767138
AN - SCOPUS:0032143616
SN - 0925-4773
VL - 76
SP - 101
EP - 115
JO - Mechanisms of Development
JF - Mechanisms of Development
IS - 1-2
ER -