TY - GEN
T1 - Rolling Circle Amplification-Coupled Nanopore for Quantification of miRNAs
AU - Dong, Ming
AU - Tang, Zifan
AU - Hicks, Steven
AU - Guan, Weihua
N1 - Publisher Copyright:
© 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.
PY - 2021
Y1 - 2021
N2 - Mild traumatic brain injury (mTBI) is the most common type of traumatic brain injury. However, the mTBI is underdiagnosed and underreported due to delayed onset of symptoms and the conventional subjective assessment. Recent findings suggest that salivary miRNA could be biomarkers for mTBI diagnosis. However, detecting and differentiating miRNAs are challenging due to the short length and high homogeneity. We developed a nanopore coupled rolling circle amplification (RCA) method to identify and quantify mTBI-related miRNAs. We believe this platform could be further developed for the clinical diagnosis of mTBI.
AB - Mild traumatic brain injury (mTBI) is the most common type of traumatic brain injury. However, the mTBI is underdiagnosed and underreported due to delayed onset of symptoms and the conventional subjective assessment. Recent findings suggest that salivary miRNA could be biomarkers for mTBI diagnosis. However, detecting and differentiating miRNAs are challenging due to the short length and high homogeneity. We developed a nanopore coupled rolling circle amplification (RCA) method to identify and quantify mTBI-related miRNAs. We believe this platform could be further developed for the clinical diagnosis of mTBI.
UR - http://www.scopus.com/inward/record.url?scp=85136966494&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85136966494&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85136966494
T3 - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 717
EP - 718
BT - MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Y2 - 10 October 2021 through 14 October 2021
ER -