TY - JOUR
T1 - Rotational manipulation of single cells and organisms using acoustic waves
AU - Ahmed, Daniel
AU - Ozcelik, Adem
AU - Bojanala, Nagagireesh
AU - Nama, Nitesh
AU - Upadhyay, Awani
AU - Chen, Yuchao
AU - Hanna-Rose, Wendy
AU - Huang, Tony Jun
PY - 2016/3/23
Y1 - 2016/3/23
N2 - The precise rotational manipulation of single cells or organisms is invaluable to many applications in biology, chemistry, physics and medicine. In this article, we describe an acoustic-based, on-chip manipulation method that can rotate single microparticles, cells and organisms. To achieve this, we trapped microbubbles within predefined sidewall microcavities inside a microchannel. In an acoustic field, trapped microbubbles were driven into oscillatory motion generating steady microvortices which were utilized to precisely rotate colloids, cells and entire organisms (that is, C. elegans). We have tested the capabilities of our method by analysing reproductive system pathologies and nervous system morphology in C. elegans. Using our device, we revealed the underlying abnormal cell fusion causing defective vulval morphology in mutant worms. Our acoustofluidic rotational manipulation (ARM) technique is an easy-to-use, compact, and biocompatible method, permitting rotation regardless of optical, magnetic or electrical properties of the sample under investigation.
AB - The precise rotational manipulation of single cells or organisms is invaluable to many applications in biology, chemistry, physics and medicine. In this article, we describe an acoustic-based, on-chip manipulation method that can rotate single microparticles, cells and organisms. To achieve this, we trapped microbubbles within predefined sidewall microcavities inside a microchannel. In an acoustic field, trapped microbubbles were driven into oscillatory motion generating steady microvortices which were utilized to precisely rotate colloids, cells and entire organisms (that is, C. elegans). We have tested the capabilities of our method by analysing reproductive system pathologies and nervous system morphology in C. elegans. Using our device, we revealed the underlying abnormal cell fusion causing defective vulval morphology in mutant worms. Our acoustofluidic rotational manipulation (ARM) technique is an easy-to-use, compact, and biocompatible method, permitting rotation regardless of optical, magnetic or electrical properties of the sample under investigation.
UR - http://www.scopus.com/inward/record.url?scp=84962261387&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84962261387&partnerID=8YFLogxK
U2 - 10.1038/ncomms11085
DO - 10.1038/ncomms11085
M3 - Article
C2 - 27004764
AN - SCOPUS:84962261387
SN - 2041-1723
VL - 7
JO - Nature communications
JF - Nature communications
M1 - 11085
ER -