SALIVA-BASED SARS-COV-2 SELF-TESTING WITH RT-LAMP IN A MOBILE DEVICE (SLIDE)

Zifan Tang, Jiarui Cui, Aneesh Kshirsagar, Tianyi Liu, Weihua Guan

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We developed a fully integrated SARS-CoV-2 nucleic acid testing (NAT) device using a self-collected saliva sample. This platform consists of a ready-to-use reagents cartridge, an easy-to-use smartphone interface, and an ultra-compact and less-expensive analyzer. Our system automatically handled the complexity of heat-inactivated sample preparation, pressure-driven sample dispensing, real-time RT-LAMP reaction and detection, and data processing, storage, and upload. With a turnaround time of ~45 minutes, our reverse transcription loop-mediated isothermal amplification (RT-LAMP) achieved the limit of detection (LoD) of 5 virus particles/μl in the saliva sample. We believe our self-testing platform will have an ongoing benefit for COVID-19 control and fighting future pandemics.

Original languageEnglish (US)
Title of host publication2022 Solid-State Sensors, Actuators and Microsystems Workshop, Hilton Head 2022
EditorsReza Ghodssi, Jenna F. Chan
PublisherTransducer Research Foundation
Pages9-12
Number of pages4
ISBN (Electronic)9781940470047
StatePublished - 2022
Event2022 Solid-State Sensors, Actuators and Microsystems Workshop, Hilton Head 2022 - Hilton Head, United States
Duration: Jun 5 2022Jun 9 2022

Publication series

Name2022 Solid-State Sensors, Actuators and Microsystems Workshop, Hilton Head 2022

Conference

Conference2022 Solid-State Sensors, Actuators and Microsystems Workshop, Hilton Head 2022
Country/TerritoryUnited States
CityHilton Head
Period6/5/226/9/22

All Science Journal Classification (ASJC) codes

  • Hardware and Architecture
  • Electrical and Electronic Engineering

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