SALIVA-BASED SARS-COV-2 SELF-TESTING WITH RT-LAMP IN A MOBILE DEVICE (SLIDE)

Zifan Tang; Jiarui Cui; Aneesh Kshirsagar; Tianyi Liu; Weihua Guan

SALIVA-BASED SARS-COV-2 SELF-TESTING WITH RT-LAMP IN A MOBILE DEVICE (SLIDE)

Zifan Tang
, Jiarui Cui
, Aneesh Kshirsagar
, Tianyi Liu
, Weihua Guan

Electrical Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Scopus citations

Abstract

We developed a fully integrated SARS-CoV-2 nucleic acid testing (NAT) device using a self-collected saliva sample. This platform consists of a ready-to-use reagents cartridge, an easy-to-use smartphone interface, and an ultra-compact and less-expensive analyzer. Our system automatically handled the complexity of heat-inactivated sample preparation, pressure-driven sample dispensing, real-time RT-LAMP reaction and detection, and data processing, storage, and upload. With a turnaround time of ~45 minutes, our reverse transcription loop-mediated isothermal amplification (RT-LAMP) achieved the limit of detection (LoD) of 5 virus particles/μl in the saliva sample. We believe our self-testing platform will have an ongoing benefit for COVID-19 control and fighting future pandemics.

Original language	English (US)
Title of host publication	2022 Solid-State Sensors, Actuators and Microsystems Workshop, Hilton Head 2022
Editors	Reza Ghodssi, Jenna F. Chan
Publisher	Transducer Research Foundation
Pages	9-12
Number of pages	4
ISBN (Electronic)	9781940470047
State	Published - 2022
Event	2022 Solid-State Sensors, Actuators and Microsystems Workshop, Hilton Head 2022 - Hilton Head, United States Duration: Jun 5 2022 → Jun 9 2022

Publication series

Name	2022 Solid-State Sensors, Actuators and Microsystems Workshop, Hilton Head 2022

Conference

Conference	2022 Solid-State Sensors, Actuators and Microsystems Workshop, Hilton Head 2022
Country/Territory	United States
City	Hilton Head
Period	6/5/22 → 6/9/22

All Science Journal Classification (ASJC) codes

Hardware and Architecture
Electrical and Electronic Engineering

Cite this

Tang, Z., Cui, J., Kshirsagar, A., Liu, T., & Guan, W. (2022). SALIVA-BASED SARS-COV-2 SELF-TESTING WITH RT-LAMP IN A MOBILE DEVICE (SLIDE). In R. Ghodssi, & J. F. Chan (Eds.), 2022 Solid-State Sensors, Actuators and Microsystems Workshop, Hilton Head 2022 (pp. 9-12). (2022 Solid-State Sensors, Actuators and Microsystems Workshop, Hilton Head 2022). Transducer Research Foundation.

Tang, Zifan ; Cui, Jiarui ; Kshirsagar, Aneesh et al. / SALIVA-BASED SARS-COV-2 SELF-TESTING WITH RT-LAMP IN A MOBILE DEVICE (SLIDE). 2022 Solid-State Sensors, Actuators and Microsystems Workshop, Hilton Head 2022. editor / Reza Ghodssi ; Jenna F. Chan. Transducer Research Foundation, 2022. pp. 9-12 (2022 Solid-State Sensors, Actuators and Microsystems Workshop, Hilton Head 2022).

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title = "SALIVA-BASED SARS-COV-2 SELF-TESTING WITH RT-LAMP IN A MOBILE DEVICE (SLIDE)",

abstract = "We developed a fully integrated SARS-CoV-2 nucleic acid testing (NAT) device using a self-collected saliva sample. This platform consists of a ready-to-use reagents cartridge, an easy-to-use smartphone interface, and an ultra-compact and less-expensive analyzer. Our system automatically handled the complexity of heat-inactivated sample preparation, pressure-driven sample dispensing, real-time RT-LAMP reaction and detection, and data processing, storage, and upload. With a turnaround time of \textasciitilde{}45 minutes, our reverse transcription loop-mediated isothermal amplification (RT-LAMP) achieved the limit of detection (LoD) of 5 virus particles/μl in the saliva sample. We believe our self-testing platform will have an ongoing benefit for COVID-19 control and fighting future pandemics.",

author = "Zifan Tang and Jiarui Cui and Aneesh Kshirsagar and Tianyi Liu and Weihua Guan",

note = "Publisher Copyright: {\textcopyright} 2022 TRF.; 2022 Solid-State Sensors, Actuators and Microsystems Workshop, Hilton Head 2022 ; Conference date: 05-06-2022 Through 09-06-2022",

year = "2022",

language = "English (US)",

series = "2022 Solid-State Sensors, Actuators and Microsystems Workshop, Hilton Head 2022",

publisher = "Transducer Research Foundation",

pages = "9--12",

editor = "Reza Ghodssi and Chan, \{Jenna F.\}",

booktitle = "2022 Solid-State Sensors, Actuators and Microsystems Workshop, Hilton Head 2022",

}

Tang, Z, Cui, J, Kshirsagar, A, Liu, T & Guan, W 2022, SALIVA-BASED SARS-COV-2 SELF-TESTING WITH RT-LAMP IN A MOBILE DEVICE (SLIDE). in R Ghodssi & JF Chan (eds), 2022 Solid-State Sensors, Actuators and Microsystems Workshop, Hilton Head 2022. 2022 Solid-State Sensors, Actuators and Microsystems Workshop, Hilton Head 2022, Transducer Research Foundation, pp. 9-12, 2022 Solid-State Sensors, Actuators and Microsystems Workshop, Hilton Head 2022, Hilton Head, United States, 6/5/22.

SALIVA-BASED SARS-COV-2 SELF-TESTING WITH RT-LAMP IN A MOBILE DEVICE (SLIDE). / Tang, Zifan; Cui, Jiarui; Kshirsagar, Aneesh et al.
2022 Solid-State Sensors, Actuators and Microsystems Workshop, Hilton Head 2022. ed. / Reza Ghodssi; Jenna F. Chan. Transducer Research Foundation, 2022. p. 9-12 (2022 Solid-State Sensors, Actuators and Microsystems Workshop, Hilton Head 2022).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - SALIVA-BASED SARS-COV-2 SELF-TESTING WITH RT-LAMP IN A MOBILE DEVICE (SLIDE)

AU - Tang, Zifan

AU - Cui, Jiarui

AU - Kshirsagar, Aneesh

AU - Liu, Tianyi

AU - Guan, Weihua

PY - 2022

Y1 - 2022

N2 - We developed a fully integrated SARS-CoV-2 nucleic acid testing (NAT) device using a self-collected saliva sample. This platform consists of a ready-to-use reagents cartridge, an easy-to-use smartphone interface, and an ultra-compact and less-expensive analyzer. Our system automatically handled the complexity of heat-inactivated sample preparation, pressure-driven sample dispensing, real-time RT-LAMP reaction and detection, and data processing, storage, and upload. With a turnaround time of ~45 minutes, our reverse transcription loop-mediated isothermal amplification (RT-LAMP) achieved the limit of detection (LoD) of 5 virus particles/μl in the saliva sample. We believe our self-testing platform will have an ongoing benefit for COVID-19 control and fighting future pandemics.

AB - We developed a fully integrated SARS-CoV-2 nucleic acid testing (NAT) device using a self-collected saliva sample. This platform consists of a ready-to-use reagents cartridge, an easy-to-use smartphone interface, and an ultra-compact and less-expensive analyzer. Our system automatically handled the complexity of heat-inactivated sample preparation, pressure-driven sample dispensing, real-time RT-LAMP reaction and detection, and data processing, storage, and upload. With a turnaround time of ~45 minutes, our reverse transcription loop-mediated isothermal amplification (RT-LAMP) achieved the limit of detection (LoD) of 5 virus particles/μl in the saliva sample. We believe our self-testing platform will have an ongoing benefit for COVID-19 control and fighting future pandemics.

UR - https://www.scopus.com/pages/publications/85172700843

UR - https://www.scopus.com/pages/publications/85172700843#tab=citedBy

M3 - Conference contribution

AN - SCOPUS:85172700843

T3 - 2022 Solid-State Sensors, Actuators and Microsystems Workshop, Hilton Head 2022

SP - 9

EP - 12

BT - 2022 Solid-State Sensors, Actuators and Microsystems Workshop, Hilton Head 2022

A2 - Ghodssi, Reza

A2 - Chan, Jenna F.

PB - Transducer Research Foundation

T2 - 2022 Solid-State Sensors, Actuators and Microsystems Workshop, Hilton Head 2022

Y2 - 5 June 2022 through 9 June 2022

ER -

SALIVA-BASED SARS-COV-2 SELF-TESTING WITH RT-LAMP IN A MOBILE DEVICE (SLIDE)

Abstract

Publication series

Conference

All Science Journal Classification (ASJC) codes

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