TY - JOUR
T1 - Sample preparation for GC/MS analysis of hydrophobic environmental contaminants by direct serum injection into restricted-access reversed-phase HPLC columns
AU - Tanaka, Nobuo
AU - Kimata, Kazuhiro
AU - Hosoya, Ken
AU - Araki, Takeo
AU - Barnhart, Elizabeth R.
AU - Alexander, Louis R.
AU - McClure, Patricia C.
AU - Lapeza, Chester
AU - Patterson, Donald G.
PY - 1995
Y1 - 1995
N2 - The extraction of polychlorinated biphenyls and polyaromatic hydrocarbons from serum was studied by reversed phase liquid chromatography using restricted-access reversed-phase packing materials. The restrictive dimensions of the hydrophobic pores of the superficially hydrophilic packings excluded serum proteins and facilitated retention of the smaller hydrophobic compounds included in the buffer-acetonitrile injection mixture. Only a relatively short time was required for analytes to be separated from less hydrophobic serum components and recovered with acetonitrile elution, providing samples for GC/MS quantification. The factors involved with injections of larger volumes of serum are addressed.
AB - The extraction of polychlorinated biphenyls and polyaromatic hydrocarbons from serum was studied by reversed phase liquid chromatography using restricted-access reversed-phase packing materials. The restrictive dimensions of the hydrophobic pores of the superficially hydrophilic packings excluded serum proteins and facilitated retention of the smaller hydrophobic compounds included in the buffer-acetonitrile injection mixture. Only a relatively short time was required for analytes to be separated from less hydrophobic serum components and recovered with acetonitrile elution, providing samples for GC/MS quantification. The factors involved with injections of larger volumes of serum are addressed.
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M3 - Article
AN - SCOPUS:0029235429
SN - 1063-5246
VL - 2
SP - 41
EP - 47
JO - Analytical methods & instrumentation
JF - Analytical methods & instrumentation
IS - 1
ER -