TY - GEN
T1 - Sample-to-answer mobile malaria molecular diagnositstic system for resource-limiting areas
AU - Choi, Gihoon
AU - Song, Daniel
AU - Shrestha, Sony
AU - Miao, Jun
AU - Cui, Liwang
AU - Guan, Weihua
PY - 2017/2/23
Y1 - 2017/2/23
N2 - This work reports a field-deployable 'sample-to-answer' molecular diagnostic system (AnyMDx) for species-specific malaria detection at the point of need. The portable nucleic acid diagnostic system uses a disposable microfluidic disc, which incorporates integrated sample preparation steps of DNA extraction, purification, elution, and amplification. Built on our previous success with highly sensitive singleplex P. falciparum detection (low detection limit of ∼0.6 parasites/μl) [1], here we report the specificity performances of AnyMDx for distinguishing two of the most common endemic malaria species (P. falciparum and P. vivax). The AnyMDx system is fully automated and delivers real-time multiplexed species-specific molecular answers directly from the raw blood samples within 40 minutes without any requirement of laboratory infrastructures.
AB - This work reports a field-deployable 'sample-to-answer' molecular diagnostic system (AnyMDx) for species-specific malaria detection at the point of need. The portable nucleic acid diagnostic system uses a disposable microfluidic disc, which incorporates integrated sample preparation steps of DNA extraction, purification, elution, and amplification. Built on our previous success with highly sensitive singleplex P. falciparum detection (low detection limit of ∼0.6 parasites/μl) [1], here we report the specificity performances of AnyMDx for distinguishing two of the most common endemic malaria species (P. falciparum and P. vivax). The AnyMDx system is fully automated and delivers real-time multiplexed species-specific molecular answers directly from the raw blood samples within 40 minutes without any requirement of laboratory infrastructures.
UR - http://www.scopus.com/inward/record.url?scp=85015736032&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85015736032&partnerID=8YFLogxK
U2 - 10.1109/MEMSYS.2017.7863449
DO - 10.1109/MEMSYS.2017.7863449
M3 - Conference contribution
AN - SCOPUS:85015736032
T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
SP - 486
EP - 489
BT - 2017 IEEE 30th International Conference on Micro Electro Mechanical Systems, MEMS 2017
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 30th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2017
Y2 - 22 January 2017 through 26 January 2017
ER -