TY - JOUR
T1 - Sample-to-answer palm-sized nucleic acid testing device towards low-cost malaria mass screening
AU - Choi, Gihoon
AU - Prince, Theodore
AU - Miao, Jun
AU - Cui, Liwang
AU - Guan, Weihua
N1 - Funding Information:
This project was partially supported by a grant from National Institutes of Health, USA ( U19AI089672 ) and a grant from National Science Foundation, USA ( ECCS-1710831 ). Support from Penn State Award ‘Materials Matter at the Human Level’ was also acknowledged. We express our gratitude to Xiaolian Li for providing malaria samples and to Jiho Noh for the 3D printing work. The following reagent was obtained through BEI Resources Repository, NIAID, NIH: Plasmodium vivax , Strain Chesson, MRA-383 and strain Achiote, MRA-369, contributed by W. E. Collins.
Publisher Copyright:
© 2018 Elsevier B.V.
PY - 2018/9/15
Y1 - 2018/9/15
N2 - The effectiveness of malaria screening and treatment highly depends on the low-cost access to the highly sensitive and specific malaria test. We report a real-time fluorescence nucleic acid testing device for malaria field detection with automated and scalable sample preparation capability. The device consists a compact analyzer and a disposable microfluidic reagent compact disc. The parasite DNA sample preparation and subsequent real-time LAMP detection were seamlessly integrated on a single microfluidic compact disc, driven by energy efficient non-centrifuge based magnetic field interactions. Each disc contains four parallel testing units which could be configured either as four identical tests or as four species-specific tests. When configured as species-specific tests, it could identify two of the most life-threatening malaria species (P. falciparum and P. vivax). The NAT device is capable of processing four samples simultaneously within 50 min turnaround time. It achieves a detection limit of ~0.5 parasites/µl for whole blood, sufficient for detecting asymptomatic parasite carriers. The combination of the sensitivity, specificity, cost, and scalable sample preparation suggests the real-time fluorescence LAMP device could be particularly useful for malaria screening in the field settings.
AB - The effectiveness of malaria screening and treatment highly depends on the low-cost access to the highly sensitive and specific malaria test. We report a real-time fluorescence nucleic acid testing device for malaria field detection with automated and scalable sample preparation capability. The device consists a compact analyzer and a disposable microfluidic reagent compact disc. The parasite DNA sample preparation and subsequent real-time LAMP detection were seamlessly integrated on a single microfluidic compact disc, driven by energy efficient non-centrifuge based magnetic field interactions. Each disc contains four parallel testing units which could be configured either as four identical tests or as four species-specific tests. When configured as species-specific tests, it could identify two of the most life-threatening malaria species (P. falciparum and P. vivax). The NAT device is capable of processing four samples simultaneously within 50 min turnaround time. It achieves a detection limit of ~0.5 parasites/µl for whole blood, sufficient for detecting asymptomatic parasite carriers. The combination of the sensitivity, specificity, cost, and scalable sample preparation suggests the real-time fluorescence LAMP device could be particularly useful for malaria screening in the field settings.
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U2 - 10.1016/j.bios.2018.05.019
DO - 10.1016/j.bios.2018.05.019
M3 - Article
C2 - 29803865
AN - SCOPUS:85047290915
SN - 0956-5663
VL - 115
SP - 83
EP - 90
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
ER -