SAP30, a component of the mSin3 corepressor complex involved in N-CoR-mediated repression by specific transcription factors

Carol D. Laherty; Andrew N. Billin; Robert M. Lavinsky; Gregory S. Yochum; Angela C. Bush; Jian Min Sun; Tina Marie Mullen; James R. Davie; David W. Rose; Christopher K. Glass; Michael G. Rosenfeld; Donald E. Ayer; Robert N. Eisenman

doi:10.1016/S1097-2765(00)80111-2

SAP30, a component of the mSin3 corepressor complex involved in N-CoR-mediated repression by specific transcription factors

Carol D. Laherty, Andrew N. Billin, Robert M. Lavinsky, Gregory S. Yochum, Angela C. Bush, Jian Min Sun, Tina Marie Mullen, James R. Davie, David W. Rose, Christopher K. Glass, Michael G. Rosenfeld, Donald E. Ayer, Robert N. Eisenman

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Abstract

The transcriptional corepressor mSin3 is found in a large multiprotein complex containing the histone deacetylases HDAC1 and HDAC2, in addition to at least five tightly associated polypeptides. We have cloned and characterized a novel component of the mSin3 complex, SAP30. SAP30 binds to mSin3 and is capable of mediating transcriptional repression via histone deacetylases. SAP30 also binds the N-CoR corepressor and is required for N-CoR-mediated repression by antagonist-bound estrogen receptor and the homeodomain protein Rpx, as well as N-CoR suppression of transactivation by the POU domain protein Pit-1. However, SAP30 is not required for N-CoR-mediated repression by unliganded retinoic acid receptor or thyroid hormone receptor, suggesting that SAP30 is involved in the functional recruitment of the mSin3-histone deacetylase complex to a specific subset of N-CoR corepressor complexes.

Original language	English (US)
Pages (from-to)	33-42
Number of pages	10
Journal	Molecular cell
Volume	2
Issue number	1
DOIs	https://doi.org/10.1016/S1097-2765(00)80111-2
State	Published - Jul 1998

All Science Journal Classification (ASJC) codes

Molecular Biology
Cell Biology

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Laherty, C. D., Billin, A. N., Lavinsky, R. M., Yochum, G. S., Bush, A. C., Sun, J. M., Mullen, T. M., Davie, J. R., Rose, D. W., Glass, C. K., Rosenfeld, M. G., Ayer, D. E., & Eisenman, R. N. (1998). SAP30, a component of the mSin3 corepressor complex involved in N-CoR-mediated repression by specific transcription factors. Molecular cell, 2(1), 33-42. https://doi.org/10.1016/S1097-2765(00)80111-2

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title = "SAP30, a component of the mSin3 corepressor complex involved in N-CoR-mediated repression by specific transcription factors",

abstract = "The transcriptional corepressor mSin3 is found in a large multiprotein complex containing the histone deacetylases HDAC1 and HDAC2, in addition to at least five tightly associated polypeptides. We have cloned and characterized a novel component of the mSin3 complex, SAP30. SAP30 binds to mSin3 and is capable of mediating transcriptional repression via histone deacetylases. SAP30 also binds the N-CoR corepressor and is required for N-CoR-mediated repression by antagonist-bound estrogen receptor and the homeodomain protein Rpx, as well as N-CoR suppression of transactivation by the POU domain protein Pit-1. However, SAP30 is not required for N-CoR-mediated repression by unliganded retinoic acid receptor or thyroid hormone receptor, suggesting that SAP30 is involved in the functional recruitment of the mSin3-histone deacetylase complex to a specific subset of N-CoR corepressor complexes.",

author = "Laherty, {Carol D.} and Billin, {Andrew N.} and Lavinsky, {Robert M.} and Yochum, {Gregory S.} and Bush, {Angela C.} and Sun, {Jian Min} and Mullen, {Tina Marie} and Davie, {James R.} and Rose, {David W.} and Glass, {Christopher K.} and Rosenfeld, {Michael G.} and Ayer, {Donald E.} and Eisenman, {Robert N.}",

note = "Funding Information: The authors thank T. Heinzel, S. Hollenberg, F. Gertler, C. Hassig, S. Schreiber, E. Seto, and Santa Cruz Biotechnology for advice or reagents, and Q. Lawrence and L. Ramos for technical assistance. We thank Y. Zhang and D. Reinberg for communicating results prior to publication. C. D. L. is a Leukemia Society of America Special Fellow, and R. N. E. is an American Cancer Society Research Professor. This work was supported by National Institutes of Health grants to R. N. E. and a Medical Reseach Council of Canada grant to J. R. D. ",

year = "1998",

month = jul,

doi = "10.1016/S1097-2765(00)80111-2",

language = "English (US)",

volume = "2",

pages = "33--42",

journal = "Molecular cell",

issn = "1097-2765",

publisher = "Cell Press",

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Laherty, CD, Billin, AN, Lavinsky, RM, Yochum, GS, Bush, AC, Sun, JM, Mullen, TM, Davie, JR, Rose, DW, Glass, CK, Rosenfeld, MG, Ayer, DE & Eisenman, RN 1998, 'SAP30, a component of the mSin3 corepressor complex involved in N-CoR-mediated repression by specific transcription factors', Molecular cell, vol. 2, no. 1, pp. 33-42. https://doi.org/10.1016/S1097-2765(00)80111-2

TY - JOUR

T1 - SAP30, a component of the mSin3 corepressor complex involved in N-CoR-mediated repression by specific transcription factors

AU - Laherty, Carol D.

AU - Billin, Andrew N.

AU - Lavinsky, Robert M.

AU - Yochum, Gregory S.

AU - Bush, Angela C.

AU - Sun, Jian Min

AU - Mullen, Tina Marie

AU - Davie, James R.

AU - Rose, David W.

AU - Glass, Christopher K.

AU - Rosenfeld, Michael G.

AU - Ayer, Donald E.

AU - Eisenman, Robert N.

N1 - Funding Information: The authors thank T. Heinzel, S. Hollenberg, F. Gertler, C. Hassig, S. Schreiber, E. Seto, and Santa Cruz Biotechnology for advice or reagents, and Q. Lawrence and L. Ramos for technical assistance. We thank Y. Zhang and D. Reinberg for communicating results prior to publication. C. D. L. is a Leukemia Society of America Special Fellow, and R. N. E. is an American Cancer Society Research Professor. This work was supported by National Institutes of Health grants to R. N. E. and a Medical Reseach Council of Canada grant to J. R. D.

PY - 1998/7

Y1 - 1998/7

N2 - The transcriptional corepressor mSin3 is found in a large multiprotein complex containing the histone deacetylases HDAC1 and HDAC2, in addition to at least five tightly associated polypeptides. We have cloned and characterized a novel component of the mSin3 complex, SAP30. SAP30 binds to mSin3 and is capable of mediating transcriptional repression via histone deacetylases. SAP30 also binds the N-CoR corepressor and is required for N-CoR-mediated repression by antagonist-bound estrogen receptor and the homeodomain protein Rpx, as well as N-CoR suppression of transactivation by the POU domain protein Pit-1. However, SAP30 is not required for N-CoR-mediated repression by unliganded retinoic acid receptor or thyroid hormone receptor, suggesting that SAP30 is involved in the functional recruitment of the mSin3-histone deacetylase complex to a specific subset of N-CoR corepressor complexes.

AB - The transcriptional corepressor mSin3 is found in a large multiprotein complex containing the histone deacetylases HDAC1 and HDAC2, in addition to at least five tightly associated polypeptides. We have cloned and characterized a novel component of the mSin3 complex, SAP30. SAP30 binds to mSin3 and is capable of mediating transcriptional repression via histone deacetylases. SAP30 also binds the N-CoR corepressor and is required for N-CoR-mediated repression by antagonist-bound estrogen receptor and the homeodomain protein Rpx, as well as N-CoR suppression of transactivation by the POU domain protein Pit-1. However, SAP30 is not required for N-CoR-mediated repression by unliganded retinoic acid receptor or thyroid hormone receptor, suggesting that SAP30 is involved in the functional recruitment of the mSin3-histone deacetylase complex to a specific subset of N-CoR corepressor complexes.

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U2 - 10.1016/S1097-2765(00)80111-2

DO - 10.1016/S1097-2765(00)80111-2

M3 - Article

C2 - 9702189

AN - SCOPUS:0032110706

SN - 1097-2765

VL - 2

SP - 33

EP - 42

JO - Molecular cell

JF - Molecular cell

IS - 1

ER -

SAP30, a component of the mSin3 corepressor complex involved in N-CoR-mediated repression by specific transcription factors

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