@article{240b0135990a4e2e8dcd93a995680095,
title = "Screening of a heptamer-type sgRNA library for potential therapeutic agents against hematological malignancies",
abstract = "tRNase-ZL-utilizing efficacious (TRUE) gene silencing is an RNA-mediated gene expression control technology that has therapeutic potential. This technology is based on the property of tRNase ZL that it can cleave any target RNA at any desired site under the direction of an appropriate artificial small guide RNA (sgRNA). To search for novel potential therapeutic sgRNAs for hematological malignancies, we screened a library composed of 156 sgRNAs, and found that 20 sgRNAs can efficiently induce apoptosis in leukemia and/or myeloma cells. Furthermore, we demonstrated that 4 of the 20 sgRNAs can reduce growth rates of HL60 cells in mouse xenograft models.",
author = "Masayuki Takahashi and Elbarbary, {Reyad A.} and Norihiro Watanabe and Atsushi Goto and Daichi Kamiya and Yoshihiro Watabe and Takayoshi Uchiyama and Miwako Narita and Masuhiro Takahashi and Yoshiaki Takahashi and Noriko Ishihara and Tatsuya Miyazawa and Tetsuo Yoshida and Mitsuoki Kawano and Masato Tamura and Masayuki Nashimoto",
note = "Funding Information: Role of the funding source . This work was supported by Adaptable and Seamless Technology Transfer Program through Target-driven R&D, Japan Science and Technology Agency , the Science Research Promotion Fund from the Promotion and Mutual Aid Corporation for Private Schools of Japan , and the JSPS KAKENHI Grant Numbers 24300342 and 11J08925 . The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. ",
year = "2014",
month = jul,
doi = "10.1016/j.leukres.2014.03.021",
language = "English (US)",
volume = "38",
pages = "808--815",
journal = "Leukemia Research",
issn = "0145-2126",
publisher = "Elsevier Limited",
number = "7",
}