Screening of a heptamer-type sgRNA library for potential therapeutic agents against hematological malignancies

Masayuki Takahashi; Reyad A. Elbarbary; Norihiro Watanabe; Atsushi Goto; Daichi Kamiya; Yoshihiro Watabe; Takayoshi Uchiyama; Miwako Narita; Masuhiro Takahashi; Yoshiaki Takahashi; Noriko Ishihara; Tatsuya Miyazawa; Tetsuo Yoshida; Mitsuoki Kawano; Masato Tamura; Masayuki Nashimoto

doi:10.1016/j.leukres.2014.03.021

Screening of a heptamer-type sgRNA library for potential therapeutic agents against hematological malignancies

Masayuki Takahashi
, Reyad A. Elbarbary
, Norihiro Watanabe
, Atsushi Goto
, Daichi Kamiya
, Yoshihiro Watabe
, Takayoshi Uchiyama
, Miwako Narita
, Masuhiro Takahashi
, Yoshiaki Takahashi
, Noriko Ishihara
, Tatsuya Miyazawa
, Tetsuo Yoshida
, Mitsuoki Kawano
, Masato Tamura
, Masayuki Nashimoto

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

tRNase-Z^L-utilizing efficacious (TRUE) gene silencing is an RNA-mediated gene expression control technology that has therapeutic potential. This technology is based on the property of tRNase Z^L that it can cleave any target RNA at any desired site under the direction of an appropriate artificial small guide RNA (sgRNA). To search for novel potential therapeutic sgRNAs for hematological malignancies, we screened a library composed of 156 sgRNAs, and found that 20 sgRNAs can efficiently induce apoptosis in leukemia and/or myeloma cells. Furthermore, we demonstrated that 4 of the 20 sgRNAs can reduce growth rates of HL60 cells in mouse xenograft models.

Original language	English (US)
Pages (from-to)	808-815
Number of pages	8
Journal	Leukemia Research
Volume	38
Issue number	7
DOIs	https://doi.org/10.1016/j.leukres.2014.03.021
State	Published - Jul 2014

All Science Journal Classification (ASJC) codes

Hematology
Oncology
Cancer Research

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10.1016/j.leukres.2014.03.021

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Takahashi, M., Elbarbary, R. A., Watanabe, N., Goto, A., Kamiya, D., Watabe, Y., Uchiyama, T., Narita, M., Takahashi, M., Takahashi, Y., Ishihara, N., Miyazawa, T., Yoshida, T., Kawano, M., Tamura, M., & Nashimoto, M. (2014). Screening of a heptamer-type sgRNA library for potential therapeutic agents against hematological malignancies. Leukemia Research, 38(7), 808-815. https://doi.org/10.1016/j.leukres.2014.03.021

@article{240b0135990a4e2e8dcd93a995680095,

title = "Screening of a heptamer-type sgRNA library for potential therapeutic agents against hematological malignancies",

abstract = "tRNase-ZL-utilizing efficacious (TRUE) gene silencing is an RNA-mediated gene expression control technology that has therapeutic potential. This technology is based on the property of tRNase ZL that it can cleave any target RNA at any desired site under the direction of an appropriate artificial small guide RNA (sgRNA). To search for novel potential therapeutic sgRNAs for hematological malignancies, we screened a library composed of 156 sgRNAs, and found that 20 sgRNAs can efficiently induce apoptosis in leukemia and/or myeloma cells. Furthermore, we demonstrated that 4 of the 20 sgRNAs can reduce growth rates of HL60 cells in mouse xenograft models.",

author = "Masayuki Takahashi and Elbarbary, \{Reyad A.\} and Norihiro Watanabe and Atsushi Goto and Daichi Kamiya and Yoshihiro Watabe and Takayoshi Uchiyama and Miwako Narita and Masuhiro Takahashi and Yoshiaki Takahashi and Noriko Ishihara and Tatsuya Miyazawa and Tetsuo Yoshida and Mitsuoki Kawano and Masato Tamura and Masayuki Nashimoto",

note = "Funding Information: Role of the funding source . This work was supported by Adaptable and Seamless Technology Transfer Program through Target-driven R\&D, Japan Science and Technology Agency , the Science Research Promotion Fund from the Promotion and Mutual Aid Corporation for Private Schools of Japan , and the JSPS KAKENHI Grant Numbers 24300342 and 11J08925 . The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. ",

year = "2014",

month = jul,

doi = "10.1016/j.leukres.2014.03.021",

language = "English (US)",

volume = "38",

pages = "808--815",

journal = "Leukemia Research",

issn = "0145-2126",

publisher = "Elsevier Ltd",

number = "7",

}

Takahashi, M, Elbarbary, RA, Watanabe, N, Goto, A, Kamiya, D, Watabe, Y, Uchiyama, T, Narita, M, Takahashi, M, Takahashi, Y, Ishihara, N, Miyazawa, T, Yoshida, T, Kawano, M, Tamura, M & Nashimoto, M 2014, 'Screening of a heptamer-type sgRNA library for potential therapeutic agents against hematological malignancies', Leukemia Research, vol. 38, no. 7, pp. 808-815. https://doi.org/10.1016/j.leukres.2014.03.021

TY - JOUR

T1 - Screening of a heptamer-type sgRNA library for potential therapeutic agents against hematological malignancies

AU - Takahashi, Masayuki

AU - Elbarbary, Reyad A.

AU - Watanabe, Norihiro

AU - Goto, Atsushi

AU - Kamiya, Daichi

AU - Watabe, Yoshihiro

AU - Uchiyama, Takayoshi

AU - Narita, Miwako

AU - Takahashi, Masuhiro

AU - Takahashi, Yoshiaki

AU - Ishihara, Noriko

AU - Miyazawa, Tatsuya

AU - Yoshida, Tetsuo

AU - Kawano, Mitsuoki

AU - Tamura, Masato

AU - Nashimoto, Masayuki

N1 - Funding Information: Role of the funding source . This work was supported by Adaptable and Seamless Technology Transfer Program through Target-driven R&D, Japan Science and Technology Agency , the Science Research Promotion Fund from the Promotion and Mutual Aid Corporation for Private Schools of Japan , and the JSPS KAKENHI Grant Numbers 24300342 and 11J08925 . The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

PY - 2014/7

Y1 - 2014/7

N2 - tRNase-ZL-utilizing efficacious (TRUE) gene silencing is an RNA-mediated gene expression control technology that has therapeutic potential. This technology is based on the property of tRNase ZL that it can cleave any target RNA at any desired site under the direction of an appropriate artificial small guide RNA (sgRNA). To search for novel potential therapeutic sgRNAs for hematological malignancies, we screened a library composed of 156 sgRNAs, and found that 20 sgRNAs can efficiently induce apoptosis in leukemia and/or myeloma cells. Furthermore, we demonstrated that 4 of the 20 sgRNAs can reduce growth rates of HL60 cells in mouse xenograft models.

AB - tRNase-ZL-utilizing efficacious (TRUE) gene silencing is an RNA-mediated gene expression control technology that has therapeutic potential. This technology is based on the property of tRNase ZL that it can cleave any target RNA at any desired site under the direction of an appropriate artificial small guide RNA (sgRNA). To search for novel potential therapeutic sgRNAs for hematological malignancies, we screened a library composed of 156 sgRNAs, and found that 20 sgRNAs can efficiently induce apoptosis in leukemia and/or myeloma cells. Furthermore, we demonstrated that 4 of the 20 sgRNAs can reduce growth rates of HL60 cells in mouse xenograft models.

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AN - SCOPUS:84902075002

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JO - Leukemia Research

JF - Leukemia Research

IS - 7

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Screening of a heptamer-type sgRNA library for potential therapeutic agents against hematological malignancies

Abstract

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