Semi-preparative asymmetrical flow field-flow fractionation: A closer look at channel dimensions and separation performance

Carmen R.M. Bria, Patrick W. Skelly, James R. Morse, Raymond E. Schaak, S. Kim Ratanathanawongs Williams

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


The design and performance of a semi-preparative asymmetrical flow field-flow fractionation (SP-AF4) channel are investigated with the objective of better understanding and exploiting the relationship between channel dimensions, sample loading, and resolution. Most size-based separations of nanometer and submicrometer particles are currently limited to analytical scale quantities (<100 μg). However, there is a strong need to fractionate and collect larger quantities so that fundamental properties of the more narrowly dispersed fractions can be studied using additional characterization methods and for subsequent applications. In this work, dimensions of the spacer that defines the form of SP-AF4 channels are varied and their performances are assessed with respect to sample focusing position and loading. Separations are performed in aqueous and organic carrier fluids. A critical evaluation of channel dimensions showed that increasing the channel breadth is a practical and effective route to maintaining separation resolution while increasing sample loads to milligram quantities. Good size resolution (∼1.0) is achieved for separations of 10 mg of 50 and 100 nm silica nanoparticles suspended in water and up to 0.6 mg of ∼10 to 35 nm inorganic hybrid nanoparticles suspended in tetrahydrofuran. This work represents important advances in the understanding of SP-AF4 separations and extends sample loading capacities in both aqueous and organic solvents.

Original languageEnglish (US)
Pages (from-to)149-157
Number of pages9
JournalJournal of Chromatography A
StatePublished - May 26 2017

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry


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