TY - JOUR
T1 - Senescence-associated gene expression during ozone-induced leaf senescence in Arabidopsis
AU - Miller, Jennifer D.
AU - Arteca, Richard N.
AU - Pell, Eva J.
PY - 1999/8
Y1 - 1999/8
N2 - The expression patterns of senescence-related genes were determined during ozone (O3) exposure in Arabidopsis. Rosettes were treated with 0.15 μL L-1 O3 for 6 h d-1 for 14 d. O3-treated leaves began to yellow after 10 d of exposure, whereas yellowing was not apparent in control leaves until d 14. Transcript levels for eight of 12 senescence related genes characterized showed induction by O3. SAG13 (senescence-associated gene), SAG21, ERD1 (early responsive to dehydration), and BCB (blue copper-binding protein) were induced within 2 to 4 d of O3 treatment; SAG18, SAG20, and ACS6 (ACC synthase) were induced within 4 to 6 d; and CCH (copper chaperone) was induced within 6 to 8 d. In contrast, levels of photosynthetic gene transcripts, rbcS (small subunit of Rubisco) and cab (chlorophyll a/b-binding protein), declined after 6 d. Other markers of natural senescence, SAG12, SAG19, MT1 (metallothionein), and Atgsr2 (glutamine synthetase), did not show enhanced transcript accumulation. When SAG12 promoter-GUS (β-glucuronidase) and SAG13 promoter-GUS transgenic plants were treated with O3, GUS activity was induced in SAG13-GUS plants after 2 d but was not detected in SAG12-GUS plants. SAG13 promoter-driven GUS activity was located throughout O3-treated leaves, whereas control leaves generally showed activity along the margins. The acceleration of leaf senescence induced by O3 is a regulated event involving many genes associated with natural senescence.
AB - The expression patterns of senescence-related genes were determined during ozone (O3) exposure in Arabidopsis. Rosettes were treated with 0.15 μL L-1 O3 for 6 h d-1 for 14 d. O3-treated leaves began to yellow after 10 d of exposure, whereas yellowing was not apparent in control leaves until d 14. Transcript levels for eight of 12 senescence related genes characterized showed induction by O3. SAG13 (senescence-associated gene), SAG21, ERD1 (early responsive to dehydration), and BCB (blue copper-binding protein) were induced within 2 to 4 d of O3 treatment; SAG18, SAG20, and ACS6 (ACC synthase) were induced within 4 to 6 d; and CCH (copper chaperone) was induced within 6 to 8 d. In contrast, levels of photosynthetic gene transcripts, rbcS (small subunit of Rubisco) and cab (chlorophyll a/b-binding protein), declined after 6 d. Other markers of natural senescence, SAG12, SAG19, MT1 (metallothionein), and Atgsr2 (glutamine synthetase), did not show enhanced transcript accumulation. When SAG12 promoter-GUS (β-glucuronidase) and SAG13 promoter-GUS transgenic plants were treated with O3, GUS activity was induced in SAG13-GUS plants after 2 d but was not detected in SAG12-GUS plants. SAG13 promoter-driven GUS activity was located throughout O3-treated leaves, whereas control leaves generally showed activity along the margins. The acceleration of leaf senescence induced by O3 is a regulated event involving many genes associated with natural senescence.
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U2 - 10.1104/pp.120.4.1015
DO - 10.1104/pp.120.4.1015
M3 - Article
C2 - 10444084
AN - SCOPUS:0033180546
SN - 0032-0889
VL - 120
SP - 1015
EP - 1023
JO - Plant physiology
JF - Plant physiology
IS - 4
ER -