Separation of fatty acid esters of retinol by high-performance liquid chromatography

A. Catharine Ross

doi:10.1016/0076-6879(90)89278-P

Separation of fatty acid esters of retinol by high-performance liquid chromatography

A. Catharine Ross

Nutritional Sciences

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Long-chain fatty acid esters of retinol comprise the major storage form of vitamin A in numerous tissues. It has long been recognized that various organs have characteristic patterns of esterified retinol and that differences may exist among animal species in the retinyl ester composition within a particular organ such as the liver. Thin-layer chromatographic methods have now been replaced by high-performance liquid chromatography (HPLC) procedures that permit excellent resolution, as well as quantification, of individual fatty acid esters of retinol. This chapter presents a brief review and comparison of four procedures that were developed for the individual analysis of retinyl ester species. All are based on similar principles and provide rather comparable separations, but they utilize different column and solvent combinations.

Original language	English (US)
Pages (from-to)	81-84
Number of pages	4
Journal	Methods in enzymology
Volume	189
Issue number	C
DOIs	https://doi.org/10.1016/0076-6879(90)89278-P
State	Published - Jan 1 1990

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Biology

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10.1016/0076-6879(90)89278-P

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title = "Separation of fatty acid esters of retinol by high-performance liquid chromatography",

abstract = "Long-chain fatty acid esters of retinol comprise the major storage form of vitamin A in numerous tissues. It has long been recognized that various organs have characteristic patterns of esterified retinol and that differences may exist among animal species in the retinyl ester composition within a particular organ such as the liver. Thin-layer chromatographic methods have now been replaced by high-performance liquid chromatography (HPLC) procedures that permit excellent resolution, as well as quantification, of individual fatty acid esters of retinol. This chapter presents a brief review and comparison of four procedures that were developed for the individual analysis of retinyl ester species. All are based on similar principles and provide rather comparable separations, but they utilize different column and solvent combinations.",

author = "{Catharine Ross}, A.",

note = "Funding Information: This work was supported by National Institutes of Health Grants HD-16484, HL-22633, DK-41479, and a Research Career Development Award, HD-00691, and by general support funds from the Howard Heinz Endowment.",

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AU - Catharine Ross, A.

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N2 - Long-chain fatty acid esters of retinol comprise the major storage form of vitamin A in numerous tissues. It has long been recognized that various organs have characteristic patterns of esterified retinol and that differences may exist among animal species in the retinyl ester composition within a particular organ such as the liver. Thin-layer chromatographic methods have now been replaced by high-performance liquid chromatography (HPLC) procedures that permit excellent resolution, as well as quantification, of individual fatty acid esters of retinol. This chapter presents a brief review and comparison of four procedures that were developed for the individual analysis of retinyl ester species. All are based on similar principles and provide rather comparable separations, but they utilize different column and solvent combinations.

AB - Long-chain fatty acid esters of retinol comprise the major storage form of vitamin A in numerous tissues. It has long been recognized that various organs have characteristic patterns of esterified retinol and that differences may exist among animal species in the retinyl ester composition within a particular organ such as the liver. Thin-layer chromatographic methods have now been replaced by high-performance liquid chromatography (HPLC) procedures that permit excellent resolution, as well as quantification, of individual fatty acid esters of retinol. This chapter presents a brief review and comparison of four procedures that were developed for the individual analysis of retinyl ester species. All are based on similar principles and provide rather comparable separations, but they utilize different column and solvent combinations.

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Separation of fatty acid esters of retinol by high-performance liquid chromatography

Abstract

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