Sequence-Specific Recognition of SARS COV-2 with Solid-State CRISPR-Cas12a-Assisted Nanopores (SCAN)

Reza Nouri, Yuqian Jiang, Zifan Tang, Xiaojun Lance Lian, Weihua Guan

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The current outbreak of the SARS-CoV-2 caused the COVID-19 disease to spread rapidly globally. Specific and sensitive detection of SARS-CoV-2 is needed to prevent the disease from spread. Here, we present a solid-state CRISPR-Cas12a-assisted nanopores (SCAN) system to detect SARS-CoV-2. We introduced a new scheme using current drops and dwell times of ssDNA reporter translocation events to estimate the cleavage activity. We validated this scheme by a statistical model approximating the reporter length distribution over the cleavage reaction. We believe that the SCAN would provide a sensitive and specific detection method for SARS-COV-2.

Original languageEnglish (US)
Title of host publicationMicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages833-834
Number of pages2
ISBN (Electronic)9781733419031
StatePublished - 2021
Event25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021 - Palm Springs, Virtual, United States
Duration: Oct 10 2021Oct 14 2021

Publication series

NameMicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Country/TerritoryUnited States
CityPalm Springs, Virtual
Period10/10/2110/14/21

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Chemical Engineering (miscellaneous)

Fingerprint

Dive into the research topics of 'Sequence-Specific Recognition of SARS COV-2 with Solid-State CRISPR-Cas12a-Assisted Nanopores (SCAN)'. Together they form a unique fingerprint.

Cite this