TY - JOUR
T1 - Shear Field-Flow Fractionation
T2 - Theoretical Basis of a New, Highly Selective Technique
AU - Giddings, J. Calvin
AU - Brantley, Susan L.
N1 - Funding Information:
This material is based upon work supported by the National Science Foundation under Grant No. CHE-8218503.
PY - 1984/8/1
Y1 - 1984/8/1
N2 - Shear field-flow fractionation (shear FFF) is described as an FFF system in which shear forces are responsible for migration perpendicular to flow. It is shown that a desirable configuration for shear FFF is a concentric cylinder system with one cylinder rotating. After providing the relevant theoretical framework of FFF, the equations of Shafer et al. describing shear migration are simplified and applied to the limiting case of very thin annular spaces to get tractable retention expressions. On this basis the maximum selectivity is predicted to be 3 or greater, a value considerably higher than that for any other macromolecular separation technique. This high selectivity is confirmed using an alternate shear migration theory developed by Tirrell et al. However, it is shown that shear FFF is only applicable to macromolecules of high molecular weight, perhaps ~107 and above. It may also be applicable to globular particles.
AB - Shear field-flow fractionation (shear FFF) is described as an FFF system in which shear forces are responsible for migration perpendicular to flow. It is shown that a desirable configuration for shear FFF is a concentric cylinder system with one cylinder rotating. After providing the relevant theoretical framework of FFF, the equations of Shafer et al. describing shear migration are simplified and applied to the limiting case of very thin annular spaces to get tractable retention expressions. On this basis the maximum selectivity is predicted to be 3 or greater, a value considerably higher than that for any other macromolecular separation technique. This high selectivity is confirmed using an alternate shear migration theory developed by Tirrell et al. However, it is shown that shear FFF is only applicable to macromolecules of high molecular weight, perhaps ~107 and above. It may also be applicable to globular particles.
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U2 - 10.1080/01496398408060666
DO - 10.1080/01496398408060666
M3 - Article
AN - SCOPUS:0021474002
SN - 0149-6395
VL - 19
SP - 631
EP - 651
JO - Separation Science and Technology
JF - Separation Science and Technology
IS - 10
ER -