TY - GEN
T1 - Shear stress increases endothelial cell-membrane fluidity
AU - Butler, Peter J.
AU - Norwich, Gerard
AU - Weinbaum, Sheldon
AU - Chien, Shu
PY - 1999/12/1
Y1 - 1999/12/1
N2 - Cell-membrane fluidity modulates protein function and mobility. Blood flow-associated shear stress induces changes in endothelial cell functions, many of which may be initiated by alterations in the plasma membrane. In this study, we quantify the effects of shear stress on endothelial cell-membrane lipid-fluidity measured by fluorescence recovery after photobleaching (FRAP). A confocal microscope was used for FRAP-measurements on DiI-stained bovine aortic endothelial cells in a parallel-plate flow chamber under static conditions, after a step-shear stress of 10 dynes/cm2 which was maintained for 15 minutes, and after the removal of shear stress. The DiI-diffusion coefficient (D) increased immediately and significantly from a pre-step value of 4.3±0.48×10-9 to 8.7±1.8×10-9 cm2/sec within 2 min after the initiation of shear stress and remained elevated thereafter. D returned to control values within 1 minute after cessation of shear stress. The novel measurements of shear-induced increases in membrane-fluidity provide important insight into the mechanisms of shear-induced protein modulation and mobilization.
AB - Cell-membrane fluidity modulates protein function and mobility. Blood flow-associated shear stress induces changes in endothelial cell functions, many of which may be initiated by alterations in the plasma membrane. In this study, we quantify the effects of shear stress on endothelial cell-membrane lipid-fluidity measured by fluorescence recovery after photobleaching (FRAP). A confocal microscope was used for FRAP-measurements on DiI-stained bovine aortic endothelial cells in a parallel-plate flow chamber under static conditions, after a step-shear stress of 10 dynes/cm2 which was maintained for 15 minutes, and after the removal of shear stress. The DiI-diffusion coefficient (D) increased immediately and significantly from a pre-step value of 4.3±0.48×10-9 to 8.7±1.8×10-9 cm2/sec within 2 min after the initiation of shear stress and remained elevated thereafter. D returned to control values within 1 minute after cessation of shear stress. The novel measurements of shear-induced increases in membrane-fluidity provide important insight into the mechanisms of shear-induced protein modulation and mobilization.
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M3 - Conference contribution
AN - SCOPUS:0033342357
SN - 0780356756
T3 - Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
BT - Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
PB - IEEE
T2 - Proceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS)
Y2 - 13 October 1999 through 16 October 1999
ER -