TY - JOUR
T1 - Short-term in vitro culture of field isolates of Plasmodium vivax using umbilical cord blood
AU - Udomsangpetch, Rachanee
AU - Somsri, Sangdao
AU - Panichakul, Tasanee
AU - Chotivanich, Kesinee
AU - Sirichaisinthop, Jeeraphat
AU - Yang, Zhaoqing
AU - Cui, Liwang
AU - Sattabongkot, Jetsumon
N1 - Funding Information:
We thank the technical assistance of the staff at the Malaria Clinics in Mae Sot, Tak, and in Prabhudhabat, Saraburi, Thailand. This work was supported by the Fogarty International Center (D43 TW006571) to L. Cui and R. Udomsangpetch, and the US Military Infectious Disease Research Program to J. Sattabongkot.
PY - 2007/3
Y1 - 2007/3
N2 - Plasmodium vivax research has been hampered by the lack of technology for culturing this parasite. Culturing P. vivax is difficult because the parasite selectively invades reticulocytes. Here we describe a modified procedure to establish and maintain short-term cultures of freshly collected P. vivax parasites using reticulocyte-enriched cord blood. Using this method, parasites could be cultured for a month. Manipulation of the culture allowed procurement of synchronized stages of the parasite. This short-term culture method can be easily adapted to study various aspects of the parasite biology.
AB - Plasmodium vivax research has been hampered by the lack of technology for culturing this parasite. Culturing P. vivax is difficult because the parasite selectively invades reticulocytes. Here we describe a modified procedure to establish and maintain short-term cultures of freshly collected P. vivax parasites using reticulocyte-enriched cord blood. Using this method, parasites could be cultured for a month. Manipulation of the culture allowed procurement of synchronized stages of the parasite. This short-term culture method can be easily adapted to study various aspects of the parasite biology.
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U2 - 10.1016/j.parint.2006.12.005
DO - 10.1016/j.parint.2006.12.005
M3 - Article
C2 - 17254835
AN - SCOPUS:33847158906
SN - 1383-5769
VL - 56
SP - 65
EP - 69
JO - Parasitology International
JF - Parasitology International
IS - 1
ER -