Short-term in vitro culture of field isolates of Plasmodium vivax using umbilical cord blood

Rachanee Udomsangpetch; Sangdao Somsri; Tasanee Panichakul; Kesinee Chotivanich; Jeeraphat Sirichaisinthop; Zhaoqing Yang; Liwang Cui; Jetsumon Sattabongkot

doi:10.1016/j.parint.2006.12.005

Short-term in vitro culture of field isolates of Plasmodium vivax using umbilical cord blood

Rachanee Udomsangpetch
, Sangdao Somsri
, Tasanee Panichakul
, Kesinee Chotivanich
, Jeeraphat Sirichaisinthop
, Zhaoqing Yang
, Liwang Cui
, Jetsumon Sattabongkot

Entomology

Research output: Contribution to journal › Article › peer-review

63 Scopus citations

Abstract

Plasmodium vivax research has been hampered by the lack of technology for culturing this parasite. Culturing P. vivax is difficult because the parasite selectively invades reticulocytes. Here we describe a modified procedure to establish and maintain short-term cultures of freshly collected P. vivax parasites using reticulocyte-enriched cord blood. Using this method, parasites could be cultured for a month. Manipulation of the culture allowed procurement of synchronized stages of the parasite. This short-term culture method can be easily adapted to study various aspects of the parasite biology.

Original language	English (US)
Pages (from-to)	65-69
Number of pages	5
Journal	Parasitology International
Volume	56
Issue number	1
DOIs	https://doi.org/10.1016/j.parint.2006.12.005
State	Published - Mar 2007

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

All Science Journal Classification (ASJC) codes

Parasitology
Infectious Diseases

Access to Document

10.1016/j.parint.2006.12.005

Cite this

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title = "Short-term in vitro culture of field isolates of Plasmodium vivax using umbilical cord blood",

abstract = "Plasmodium vivax research has been hampered by the lack of technology for culturing this parasite. Culturing P. vivax is difficult because the parasite selectively invades reticulocytes. Here we describe a modified procedure to establish and maintain short-term cultures of freshly collected P. vivax parasites using reticulocyte-enriched cord blood. Using this method, parasites could be cultured for a month. Manipulation of the culture allowed procurement of synchronized stages of the parasite. This short-term culture method can be easily adapted to study various aspects of the parasite biology.",

author = "Rachanee Udomsangpetch and Sangdao Somsri and Tasanee Panichakul and Kesinee Chotivanich and Jeeraphat Sirichaisinthop and Zhaoqing Yang and Liwang Cui and Jetsumon Sattabongkot",

note = "Funding Information: We thank the technical assistance of the staff at the Malaria Clinics in Mae Sot, Tak, and in Prabhudhabat, Saraburi, Thailand. This work was supported by the Fogarty International Center (D43 TW006571) to L. Cui and R. Udomsangpetch, and the US Military Infectious Disease Research Program to J. Sattabongkot.",

year = "2007",

month = mar,

doi = "10.1016/j.parint.2006.12.005",

language = "English (US)",

volume = "56",

pages = "65--69",

journal = "Parasitology International",

issn = "1383-5769",

publisher = "Elsevier Ireland Ltd",

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}

TY - JOUR

T1 - Short-term in vitro culture of field isolates of Plasmodium vivax using umbilical cord blood

AU - Udomsangpetch, Rachanee

AU - Somsri, Sangdao

AU - Panichakul, Tasanee

AU - Chotivanich, Kesinee

AU - Sirichaisinthop, Jeeraphat

AU - Yang, Zhaoqing

AU - Cui, Liwang

AU - Sattabongkot, Jetsumon

N1 - Funding Information: We thank the technical assistance of the staff at the Malaria Clinics in Mae Sot, Tak, and in Prabhudhabat, Saraburi, Thailand. This work was supported by the Fogarty International Center (D43 TW006571) to L. Cui and R. Udomsangpetch, and the US Military Infectious Disease Research Program to J. Sattabongkot.

PY - 2007/3

Y1 - 2007/3

N2 - Plasmodium vivax research has been hampered by the lack of technology for culturing this parasite. Culturing P. vivax is difficult because the parasite selectively invades reticulocytes. Here we describe a modified procedure to establish and maintain short-term cultures of freshly collected P. vivax parasites using reticulocyte-enriched cord blood. Using this method, parasites could be cultured for a month. Manipulation of the culture allowed procurement of synchronized stages of the parasite. This short-term culture method can be easily adapted to study various aspects of the parasite biology.

AB - Plasmodium vivax research has been hampered by the lack of technology for culturing this parasite. Culturing P. vivax is difficult because the parasite selectively invades reticulocytes. Here we describe a modified procedure to establish and maintain short-term cultures of freshly collected P. vivax parasites using reticulocyte-enriched cord blood. Using this method, parasites could be cultured for a month. Manipulation of the culture allowed procurement of synchronized stages of the parasite. This short-term culture method can be easily adapted to study various aspects of the parasite biology.

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DO - 10.1016/j.parint.2006.12.005

M3 - Article

C2 - 17254835

AN - SCOPUS:33847158906

SN - 1383-5769

VL - 56

SP - 65

EP - 69

JO - Parasitology International

JF - Parasitology International

IS - 1

ER -

Short-term in vitro culture of field isolates of Plasmodium vivax using umbilical cord blood

Abstract

UN SDGs

All Science Journal Classification (ASJC) codes

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