TY - JOUR
T1 - Short‐term cell‐attachment rates
T2 - A surface‐sensitives test of cell–substrate compatibility
AU - Vogler, E. A.
AU - Bussian, R. W.
PY - 1987/10
Y1 - 1987/10
N2 - Mechanisms of cell deposition from a sessile liquid phase and adherence to various plastic substrates have been investigated by measurement of short‐term (<120 min) cell‐attachment rates. Sigmoidal attachment‐rate curves were fit with a three‐parameter variant of a logistic equation to quantify parameters related to initial rate and equilibrium‐adherence. For substrates on which cell adhesion was low, initial rates were estimated from slopes of linear bestfit equations. Average variations in adherence parameters for three cell lines, MDCK (epithelioid), VERO, and AHL‐1 (fibroblastic) to tissue‐culture grade polystyrene dishes were less than 10% (standard‐error‐of‐mean/mean × 100) over extended periods of more than two months, so that attachment measurements could be repetitively applied with the same cell stocks continuously subcultured in the laboratory. Developed techniques were applied to a variety of plastics and the results tabulated. Comparison of adherence parameters for ionomer and polyethylene films, both with and without adsorbed fetal‐bovineserum proteins, demonstrated that surface carboxyl groups were important in protein adsorption and cellular adherence. Attachment rates of MDCK to polystyrene were dependent on starting cell number whereas equilibrium adherence did not vary significantly over a wide range of inoculum concentrations. It was concluded from theoretical considerations that initial rates of MDCK attachment were sensitive to and dependent on electrostatic barriers to formation of close substrate contacts whereas equilibrium‐adherence levels were controlled by short‐range forces such as interfacial energies and formation of receptor‐ligand complexes.
AB - Mechanisms of cell deposition from a sessile liquid phase and adherence to various plastic substrates have been investigated by measurement of short‐term (<120 min) cell‐attachment rates. Sigmoidal attachment‐rate curves were fit with a three‐parameter variant of a logistic equation to quantify parameters related to initial rate and equilibrium‐adherence. For substrates on which cell adhesion was low, initial rates were estimated from slopes of linear bestfit equations. Average variations in adherence parameters for three cell lines, MDCK (epithelioid), VERO, and AHL‐1 (fibroblastic) to tissue‐culture grade polystyrene dishes were less than 10% (standard‐error‐of‐mean/mean × 100) over extended periods of more than two months, so that attachment measurements could be repetitively applied with the same cell stocks continuously subcultured in the laboratory. Developed techniques were applied to a variety of plastics and the results tabulated. Comparison of adherence parameters for ionomer and polyethylene films, both with and without adsorbed fetal‐bovineserum proteins, demonstrated that surface carboxyl groups were important in protein adsorption and cellular adherence. Attachment rates of MDCK to polystyrene were dependent on starting cell number whereas equilibrium adherence did not vary significantly over a wide range of inoculum concentrations. It was concluded from theoretical considerations that initial rates of MDCK attachment were sensitive to and dependent on electrostatic barriers to formation of close substrate contacts whereas equilibrium‐adherence levels were controlled by short‐range forces such as interfacial energies and formation of receptor‐ligand complexes.
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U2 - 10.1002/jbm.820211004
DO - 10.1002/jbm.820211004
M3 - Article
C2 - 3693384
AN - SCOPUS:0023434264
SN - 0021-9304
VL - 21
SP - 1197
EP - 1211
JO - Journal of Biomedical Materials Research
JF - Journal of Biomedical Materials Research
IS - 10
ER -