Simple method for determining nucleobase pKa values by indirect labeling and demonstration of a pKa of neutrality in dsDNA

Ellen M. Moody; Trevor S. Brown; Philip C. Bevilacqua

doi:10.1021/ja047362h

Simple method for determining nucleobase pK_a values by indirect labeling and demonstration of a pK_a of neutrality in dsDNA

Ellen M. Moody, Trevor S. Brown, Philip C. Bevilacqua

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27 Scopus citations

Abstract

Phosphorothioates were substituted into double-stranded DNA to study protonated Class I A⁺·C basepairs by ³¹P NMR. The method was effective in reporting the A⁺·C pK_a. pK_a values near 7.0 were found with optimal nearest-neighbor partners. Such pK_a values could expand the catalytic repertoire of nucleic acids.

Original language	English (US)
Pages (from-to)	10200-10201
Number of pages	2
Journal	Journal of the American Chemical Society
Volume	126
Issue number	33
DOIs	https://doi.org/10.1021/ja047362h
State	Published - Aug 25 2004

All Science Journal Classification (ASJC) codes

Catalysis
General Chemistry
Biochemistry
Colloid and Surface Chemistry

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10.1021/ja047362h

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title = "Simple method for determining nucleobase pKa values by indirect labeling and demonstration of a pKa of neutrality in dsDNA",

abstract = "Phosphorothioates were substituted into double-stranded DNA to study protonated Class I A+·C basepairs by 31P NMR. The method was effective in reporting the A+·C pKa. pKa values near 7.0 were found with optimal nearest-neighbor partners. Such pKa values could expand the catalytic repertoire of nucleic acids.",

author = "Moody, {Ellen M.} and Brown, {Trevor S.} and Bevilacqua, {Philip C.}",

year = "2004",

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journal = "Journal of the American Chemical Society",

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AU - Moody, Ellen M.

AU - Brown, Trevor S.

AU - Bevilacqua, Philip C.

PY - 2004/8/25

Y1 - 2004/8/25

N2 - Phosphorothioates were substituted into double-stranded DNA to study protonated Class I A+·C basepairs by 31P NMR. The method was effective in reporting the A+·C pKa. pKa values near 7.0 were found with optimal nearest-neighbor partners. Such pKa values could expand the catalytic repertoire of nucleic acids.

AB - Phosphorothioates were substituted into double-stranded DNA to study protonated Class I A+·C basepairs by 31P NMR. The method was effective in reporting the A+·C pKa. pKa values near 7.0 were found with optimal nearest-neighbor partners. Such pKa values could expand the catalytic repertoire of nucleic acids.

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JF - Journal of the American Chemical Society

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Simple method for determining nucleobase pK_a values by indirect labeling and demonstration of a pK_a of neutrality in dsDNA

Abstract

All Science Journal Classification (ASJC) codes

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