Simple Polymethylglutarimide Microfluidic Channels With Hydrogel-Assisted Fluid Exchange

Akanksha V. Gupta, Alyssa N. Brigeman, Esther W. Gomez, Thomas N. Jackson

Research output: Contribution to journalArticlepeer-review


We present an experimental protocol for fabricating enclosed microfluidic channels using polymethylglutarimide (PMGI). PMGI is optically transparent, biocompatible, and can be used to readily fabricate micrometer-scale lateral and vertical dimension channels using conventional photolithography. The low auto-fluorescence intensity of PMGI facilitates imaging of analytes without interference. The hydrophilicity of PMGI allows fluid exchange in micrometer-scale channels using a hydrogel as an interface without an external pump. As a demonstration, we assemble fluorescently-labeled lipid bilayers in PMGI microfluidic channels and show that PMGI has negligible auto-fluorescence intensity compared to the lipid bilayer. PMGI channels together with hydrogel-assisted fluidic exchange provides a simple approach to fabricate micrometer and sub-micrometer scale fluidic channels for optofluidics, molecular biology, and other medical diagnostic and sensing applications.

Original languageEnglish (US)
Pages (from-to)97-101
Number of pages5
JournalIEEE Transactions on Nanobioscience
Issue number2
StatePublished - Apr 2018

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering
  • Pharmaceutical Science
  • Computer Science Applications
  • Electrical and Electronic Engineering


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