TY - JOUR
T1 - Simple Polymethylglutarimide Microfluidic Channels With Hydrogel-Assisted Fluid Exchange
AU - Gupta, Akanksha V.
AU - Brigeman, Alyssa N.
AU - Gomez, Esther W.
AU - Jackson, Thomas N.
N1 - Funding Information:
Manuscript received February 22, 2018; revised April 12, 2018; accepted April 19, 2018. Date of publication April 25, 2018; date of current version June 1, 2018. This work was supported by the NIH under Grant R01 GM100076-04. (Corresponding author: Akanksha V. Gupta.) A. V. Gupta is with the Department of Chemical Engineering, Pennsylvania State University, University Park, PA 16802 USA (e-mail: [email protected]).
Publisher Copyright:
© 2002-2011 IEEE.
PY - 2018/4
Y1 - 2018/4
N2 - We present an experimental protocol for fabricating enclosed microfluidic channels using polymethylglutarimide (PMGI). PMGI is optically transparent, biocompatible, and can be used to readily fabricate micrometer-scale lateral and vertical dimension channels using conventional photolithography. The low auto-fluorescence intensity of PMGI facilitates imaging of analytes without interference. The hydrophilicity of PMGI allows fluid exchange in micrometer-scale channels using a hydrogel as an interface without an external pump. As a demonstration, we assemble fluorescently-labeled lipid bilayers in PMGI microfluidic channels and show that PMGI has negligible auto-fluorescence intensity compared to the lipid bilayer. PMGI channels together with hydrogel-assisted fluidic exchange provides a simple approach to fabricate micrometer and sub-micrometer scale fluidic channels for optofluidics, molecular biology, and other medical diagnostic and sensing applications.
AB - We present an experimental protocol for fabricating enclosed microfluidic channels using polymethylglutarimide (PMGI). PMGI is optically transparent, biocompatible, and can be used to readily fabricate micrometer-scale lateral and vertical dimension channels using conventional photolithography. The low auto-fluorescence intensity of PMGI facilitates imaging of analytes without interference. The hydrophilicity of PMGI allows fluid exchange in micrometer-scale channels using a hydrogel as an interface without an external pump. As a demonstration, we assemble fluorescently-labeled lipid bilayers in PMGI microfluidic channels and show that PMGI has negligible auto-fluorescence intensity compared to the lipid bilayer. PMGI channels together with hydrogel-assisted fluidic exchange provides a simple approach to fabricate micrometer and sub-micrometer scale fluidic channels for optofluidics, molecular biology, and other medical diagnostic and sensing applications.
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U2 - 10.1109/TNB.2018.2829878
DO - 10.1109/TNB.2018.2829878
M3 - Article
C2 - 29870332
AN - SCOPUS:85046001456
SN - 1536-1241
VL - 17
SP - 97
EP - 101
JO - IEEE Transactions on Nanobioscience
JF - IEEE Transactions on Nanobioscience
IS - 2
ER -