Simultaneous Counting of Molecules in the Halo and Dense-Core of Nanovesicles by Regulating Dynamics of Vesicle Opening

Xiulan He, Andrew G. Ewing

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

We report the discovery that in the presence of chaotropic anions (SCN) the opening of nanometer biological vesicles at an electrified interface often becomes a two-step process (around 30 % doublet peaks). We have then used this to independently count molecules in each subvesicular compartment, the halo and protein dense-core, and the fraction of catecholamine binding to the dense-core is 68 %. Moreover, we differentiated two distinct populations of large dense-core vesicles (LDCVs) and quantified their content, which might correspond to immature (43 %) and mature (30 %) LDCVs, to reveal differences in their biogenesis. We speculate this is caused by an increase in the electrostatic attraction between protonated catecholamine and the negatively charged dense-core following adsorption of SCN.

Original languageEnglish (US)
Article numbere202116217
JournalAngewandte Chemie - International Edition
Volume61
Issue number15
DOIs
StatePublished - Apr 4 2022

All Science Journal Classification (ASJC) codes

  • Catalysis
  • General Chemistry

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