TY - JOUR
T1 - Simultaneous determination of thioridazine and its S-oxidized and N-demethylated metabolites using high-performance liquid chromatography on radially compressed silica
AU - Kilts, Clinton D.
AU - Patrick, Kennerly S.
AU - Breese, George R.
AU - Mailman, Richard B.
N1 - Funding Information:
This work was supported by PHS Grants Nos. HD-10570 and HD-03110. C.D.K. holds a postdoctoral National Research Service Award (MH-07926), and R.B.M. is a Young Investigator Awardee (ES-02087). The authors wish to thank Faygele ben Miriam for assistance in the preparation of this manuscript_ Vie also grateWy acknowledge Dr. J. Garbutt for the collection of clinical samples_
PY - 1982/9/10
Y1 - 1982/9/10
N2 - A method for simultaneously quantifying thioridazine, northioridazine, thioridazine-2-sulfoxide, thioridazine-2-sulfone and thioridazine-5-oxide in serum and plasma is described. Following solvent extraction these compounds were separated by high-performance liquid chromatography on radially compressed silica gel and detected by UV absorbance at 254 nm. Chromatography time is less than 7 min. The relative retention of these compounds as a function of the methanol and methylamine content of the mobile phase is discussed. Practical limits of detection, based upon an assayed plasma or serum volume of 1 ml, were 20 ng/ml for thioridazine-5-oxide and 10 ng/ml for the other compounds. The coefficient of variation for all compounds was less than 13%. The method is compared with more conventional high-performance liquid chromatographic and gas chromatographic methodology.
AB - A method for simultaneously quantifying thioridazine, northioridazine, thioridazine-2-sulfoxide, thioridazine-2-sulfone and thioridazine-5-oxide in serum and plasma is described. Following solvent extraction these compounds were separated by high-performance liquid chromatography on radially compressed silica gel and detected by UV absorbance at 254 nm. Chromatography time is less than 7 min. The relative retention of these compounds as a function of the methanol and methylamine content of the mobile phase is discussed. Practical limits of detection, based upon an assayed plasma or serum volume of 1 ml, were 20 ng/ml for thioridazine-5-oxide and 10 ng/ml for the other compounds. The coefficient of variation for all compounds was less than 13%. The method is compared with more conventional high-performance liquid chromatographic and gas chromatographic methodology.
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U2 - 10.1016/S0378-4347(00)81862-9
DO - 10.1016/S0378-4347(00)81862-9
M3 - Article
C2 - 6290516
AN - SCOPUS:0020371455
SN - 0378-4347
VL - 231
SP - 377
EP - 391
JO - Journal of Chromatography B: Biomedical Sciences and Applications
JF - Journal of Chromatography B: Biomedical Sciences and Applications
IS - 2
ER -