Simultaneous determination of thioridazine and its S-oxidized and N-demethylated metabolites using high-performance liquid chromatography on radially compressed silica

Clinton D. Kilts; Kennerly S. Patrick; George R. Breese; Richard B. Mailman

doi:10.1016/S0378-4347(00)81862-9

Simultaneous determination of thioridazine and its S-oxidized and N-demethylated metabolites using high-performance liquid chromatography on radially compressed silica

Clinton D. Kilts
, Kennerly S. Patrick
, George R. Breese
, Richard B. Mailman

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

A method for simultaneously quantifying thioridazine, northioridazine, thioridazine-2-sulfoxide, thioridazine-2-sulfone and thioridazine-5-oxide in serum and plasma is described. Following solvent extraction these compounds were separated by high-performance liquid chromatography on radially compressed silica gel and detected by UV absorbance at 254 nm. Chromatography time is less than 7 min. The relative retention of these compounds as a function of the methanol and methylamine content of the mobile phase is discussed. Practical limits of detection, based upon an assayed plasma or serum volume of 1 ml, were 20 ng/ml for thioridazine-5-oxide and 10 ng/ml for the other compounds. The coefficient of variation for all compounds was less than 13%. The method is compared with more conventional high-performance liquid chromatographic and gas chromatographic methodology.

Original language	English (US)
Pages (from-to)	377-391
Number of pages	15
Journal	Journal of Chromatography B: Biomedical Sciences and Applications
Volume	231
Issue number	2
DOIs	https://doi.org/10.1016/S0378-4347(00)81862-9
State	Published - Sep 10 1982

All Science Journal Classification (ASJC) codes

General Chemistry

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@article{e41a3316565049f6af1d1ee9565e0cce,

title = "Simultaneous determination of thioridazine and its S-oxidized and N-demethylated metabolites using high-performance liquid chromatography on radially compressed silica",

abstract = "A method for simultaneously quantifying thioridazine, northioridazine, thioridazine-2-sulfoxide, thioridazine-2-sulfone and thioridazine-5-oxide in serum and plasma is described. Following solvent extraction these compounds were separated by high-performance liquid chromatography on radially compressed silica gel and detected by UV absorbance at 254 nm. Chromatography time is less than 7 min. The relative retention of these compounds as a function of the methanol and methylamine content of the mobile phase is discussed. Practical limits of detection, based upon an assayed plasma or serum volume of 1 ml, were 20 ng/ml for thioridazine-5-oxide and 10 ng/ml for the other compounds. The coefficient of variation for all compounds was less than 13\%. The method is compared with more conventional high-performance liquid chromatographic and gas chromatographic methodology.",

author = "Kilts, \{Clinton D.\} and Patrick, \{Kennerly S.\} and Breese, \{George R.\} and Mailman, \{Richard B.\}",

note = "Funding Information: This work was supported by PHS Grants Nos. HD-10570 and HD-03110. C.D.K. holds a postdoctoral National Research Service Award (MH-07926), and R.B.M. is a Young Investigator Awardee (ES-02087). The authors wish to thank Faygele ben Miriam for assistance in the preparation of this manuscript\_ Vie also grateWy acknowledge Dr. J. Garbutt for the collection of clinical samples\_",

year = "1982",

month = sep,

day = "10",

doi = "10.1016/S0378-4347(00)81862-9",

language = "English (US)",

volume = "231",

pages = "377--391",

journal = "Journal of Chromatography B: Biomedical Sciences and Applications",

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publisher = "Elsevier BV",

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}

Simultaneous determination of thioridazine and its S-oxidized and N-demethylated metabolites using high-performance liquid chromatography on radially compressed silica. / Kilts, Clinton D.; Patrick, Kennerly S.; Breese, George R. et al.
In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 231, No. 2, 10.09.1982, p. 377-391.

Research output: Contribution to journal › Article › peer-review

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T1 - Simultaneous determination of thioridazine and its S-oxidized and N-demethylated metabolites using high-performance liquid chromatography on radially compressed silica

AU - Kilts, Clinton D.

AU - Patrick, Kennerly S.

AU - Breese, George R.

AU - Mailman, Richard B.

N1 - Funding Information: This work was supported by PHS Grants Nos. HD-10570 and HD-03110. C.D.K. holds a postdoctoral National Research Service Award (MH-07926), and R.B.M. is a Young Investigator Awardee (ES-02087). The authors wish to thank Faygele ben Miriam for assistance in the preparation of this manuscript_ Vie also grateWy acknowledge Dr. J. Garbutt for the collection of clinical samples_

PY - 1982/9/10

Y1 - 1982/9/10

N2 - A method for simultaneously quantifying thioridazine, northioridazine, thioridazine-2-sulfoxide, thioridazine-2-sulfone and thioridazine-5-oxide in serum and plasma is described. Following solvent extraction these compounds were separated by high-performance liquid chromatography on radially compressed silica gel and detected by UV absorbance at 254 nm. Chromatography time is less than 7 min. The relative retention of these compounds as a function of the methanol and methylamine content of the mobile phase is discussed. Practical limits of detection, based upon an assayed plasma or serum volume of 1 ml, were 20 ng/ml for thioridazine-5-oxide and 10 ng/ml for the other compounds. The coefficient of variation for all compounds was less than 13%. The method is compared with more conventional high-performance liquid chromatographic and gas chromatographic methodology.

AB - A method for simultaneously quantifying thioridazine, northioridazine, thioridazine-2-sulfoxide, thioridazine-2-sulfone and thioridazine-5-oxide in serum and plasma is described. Following solvent extraction these compounds were separated by high-performance liquid chromatography on radially compressed silica gel and detected by UV absorbance at 254 nm. Chromatography time is less than 7 min. The relative retention of these compounds as a function of the methanol and methylamine content of the mobile phase is discussed. Practical limits of detection, based upon an assayed plasma or serum volume of 1 ml, were 20 ng/ml for thioridazine-5-oxide and 10 ng/ml for the other compounds. The coefficient of variation for all compounds was less than 13%. The method is compared with more conventional high-performance liquid chromatographic and gas chromatographic methodology.

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Simultaneous determination of thioridazine and its S-oxidized and N-demethylated metabolites using high-performance liquid chromatography on radially compressed silica

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