Abstract
In this study, molecular beacons have been successfully transfected into living cells with high efficiency (80%∼90%) inside a microfluidic system. This will enable a sensitive and specific method to monitor gene expression dynamics in single living cells. Fluorescence conjugated transfection reagent was adopted to investigate the transfection dynamics of molecular beacons. The development of microfluidic systems has facilitated real-time monitoring of individual cells, which revealed the transfection dynamics of molecular beacons at the single cell level.
| Original language | English (US) |
|---|---|
| Pages | 969-971 |
| Number of pages | 3 |
| State | Published - 2006 |
| Event | 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan Duration: Nov 5 2006 → Nov 9 2006 |
Other
| Other | 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 |
|---|---|
| Country/Territory | Japan |
| City | Tokyo |
| Period | 11/5/06 → 11/9/06 |
All Science Journal Classification (ASJC) codes
- Chemical Engineering (miscellaneous)
- Bioengineering