TY - JOUR
T1 - Single-molecule analysis of RNA polymerase transcription
AU - Bai, Lu
AU - Santangelo, Thomas J.
AU - Wang, Michelle D.
PY - 2006
Y1 - 2006
N2 - The kinetics and mechanisms of transcription are now being investigated by a repertoire of single-molecule techniques, including optical and magnetic tweezers, high-sensitivity fluorescence techniques, and atomic force microscopy. Single-molecule techniques complement traditional biochemical and crystallographic approaches, are capable of detecting the motions and dynamics of individual RNAP molecules and transcription complexes in real time, and make it possible to directly measure RNAP binding to and unwinding of template DNA, as well as RNAP translocation along the DNA during transcript synthesis.
AB - The kinetics and mechanisms of transcription are now being investigated by a repertoire of single-molecule techniques, including optical and magnetic tweezers, high-sensitivity fluorescence techniques, and atomic force microscopy. Single-molecule techniques complement traditional biochemical and crystallographic approaches, are capable of detecting the motions and dynamics of individual RNAP molecules and transcription complexes in real time, and make it possible to directly measure RNAP binding to and unwinding of template DNA, as well as RNAP translocation along the DNA during transcript synthesis.
UR - http://www.scopus.com/inward/record.url?scp=33745041963&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33745041963&partnerID=8YFLogxK
U2 - 10.1146/annurev.biophys.35.010406.150153
DO - 10.1146/annurev.biophys.35.010406.150153
M3 - Review article
C2 - 16689640
AN - SCOPUS:33745041963
SN - 1056-8700
VL - 35
SP - 343
EP - 360
JO - Annual Review of Biophysics and Biomolecular Structure
JF - Annual Review of Biophysics and Biomolecular Structure
ER -