TY - JOUR
T1 - Single-molecule studies of DNA replisome function
AU - Perumal, Senthil K.
AU - Yue, Hongjun
AU - Hu, Zhenxin
AU - Spiering, Michelle M.
AU - Benkovic, Stephen J.
N1 - Funding Information:
This work is supported by National Institute of Health Grant GM013306 to S.J.B. We would like to thank Nathan Tanner and Prof. Antoine van Oijen for providing us with their high-resolution figures.
PY - 2010/5
Y1 - 2010/5
N2 - Fast and accurate replication of DNA is accomplished by the interactions of multiple proteins in the dynamic DNA replisome. The DNA replisome effectively coordinates the leading and lagging strand synthesis of DNA. These complex, yet elegantly organized, molecular machines have been studied extensively by kinetic and structural methods to provide an in-depth understanding of the mechanism of DNA replication. Owing to averaging of observables, unique dynamic information of the biochemical pathways and reactions is concealed in conventional ensemble methods. However, recent advances in the rapidly expanding field of single-molecule analyses to study single biomolecules offer opportunities to probe and understand the dynamic processes involved in large biomolecular complexes such as replisomes. This review will focus on the recent developments in the biochemistry and biophysics of DNA replication employing single-molecule techniques and the insights provided by these methods towards a better understanding of the intricate mechanisms of DNA replication.
AB - Fast and accurate replication of DNA is accomplished by the interactions of multiple proteins in the dynamic DNA replisome. The DNA replisome effectively coordinates the leading and lagging strand synthesis of DNA. These complex, yet elegantly organized, molecular machines have been studied extensively by kinetic and structural methods to provide an in-depth understanding of the mechanism of DNA replication. Owing to averaging of observables, unique dynamic information of the biochemical pathways and reactions is concealed in conventional ensemble methods. However, recent advances in the rapidly expanding field of single-molecule analyses to study single biomolecules offer opportunities to probe and understand the dynamic processes involved in large biomolecular complexes such as replisomes. This review will focus on the recent developments in the biochemistry and biophysics of DNA replication employing single-molecule techniques and the insights provided by these methods towards a better understanding of the intricate mechanisms of DNA replication.
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U2 - 10.1016/j.bbapap.2009.07.022
DO - 10.1016/j.bbapap.2009.07.022
M3 - Review article
C2 - 19665592
AN - SCOPUS:77949567580
SN - 1570-9639
VL - 1804
SP - 1094
EP - 1112
JO - Biochimica et Biophysica Acta - Proteins and Proteomics
JF - Biochimica et Biophysica Acta - Proteins and Proteomics
IS - 5
ER -