Single tree genetic linkage mapping in conifers using haploid DNA from megagametophytes

Lomas K. Tulsieram; Jeffrey C. Glaubitz; Gyula Kiss; John E. Carlson

doi:10.1038/nbt0692-686

Single tree genetic linkage mapping in conifers using haploid DNA from megagametophytes

Lomas K. Tulsieram, Jeffrey C. Glaubitz, Gyula Kiss, John E. Carlson

Ecosystem Science and Management

Research output: Contribution to journal › Article › peer-review

139 Scopus citations

Abstract

We report a unique application of the Random Amplified Polymorphic DNA (RAPD) technique for genetic linkage mapping of a single spruce tree using haploid DNA from megagametophyte tissue of individual seeds. Sixty-one segregating loci were analysed for reproducibility, inheritance and linkage. Forty-seven of the 61 markers were distributed into 12 linkage groups and covered 873.8 cM. The 14 markers not associated with any linkage group should be assigned to linkage groups as more markers are added to the map. This new approach quickly provides molecular genetic markers that are simple to evaluate for constructing genetic linkage maps and for other related genetic studies in forest tree species.

Original language	English (US)
Pages (from-to)	686-690
Number of pages	5
Journal	Bio/Technology
Volume	10
Issue number	6
DOIs	https://doi.org/10.1038/nbt0692-686
State	Published - Jun 1992

All Science Journal Classification (ASJC) codes

Biotechnology

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abstract = "We report a unique application of the Random Amplified Polymorphic DNA (RAPD) technique for genetic linkage mapping of a single spruce tree using haploid DNA from megagametophyte tissue of individual seeds. Sixty-one segregating loci were analysed for reproducibility, inheritance and linkage. Forty-seven of the 61 markers were distributed into 12 linkage groups and covered 873.8 cM. The 14 markers not associated with any linkage group should be assigned to linkage groups as more markers are added to the map. This new approach quickly provides molecular genetic markers that are simple to evaluate for constructing genetic linkage maps and for other related genetic studies in forest tree species.",

author = "Tulsieram, {Lomas K.} and Glaubitz, {Jeffrey C.} and Gyula Kiss and Carlson, {John E.}",

year = "1992",

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AU - Carlson, John E.

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AB - We report a unique application of the Random Amplified Polymorphic DNA (RAPD) technique for genetic linkage mapping of a single spruce tree using haploid DNA from megagametophyte tissue of individual seeds. Sixty-one segregating loci were analysed for reproducibility, inheritance and linkage. Forty-seven of the 61 markers were distributed into 12 linkage groups and covered 873.8 cM. The 14 markers not associated with any linkage group should be assigned to linkage groups as more markers are added to the map. This new approach quickly provides molecular genetic markers that are simple to evaluate for constructing genetic linkage maps and for other related genetic studies in forest tree species.

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Single tree genetic linkage mapping in conifers using haploid DNA from megagametophytes

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