Site-Directed Mutagenesis of a Catalytic Antibody: An Arginine and a Histidine Residue Play Key Roles

Keyphrases

• Arginine 100%
• Site-directed mutagenesis 100%
• Catalytic Activity 100%
• Histidine Residue 100%
• Catalytic Antibodies 100%
• Acyl 50%
• Nucleophile 50%
• Binding Activity 25%
• Hydroxide 25%
• Substrate Binding 25%
• Side Chain 25%
• Kinetic Study 25%
• Mutant Protein 25%
• Transition State 25%
• Computer Model 25%
• Expression System 25%
• Computer Modeling 25%
• Catalytic Efficiency 25%
• Ligand Binding 25%
• Structural Role 25%
• Imidazole 25%
• Acid Hydrolysis 25%
• Carbonyl Group 25%
• Engineered Escherichia Coli 25%
• Oxyanion Hole 25%
• Single-chain Antibody 25%

Biochemistry, Genetics and Molecular Biology

• Site-Directed Mutagenesis 100%
• Histidine 100%
• Enzymatic Activity 100%
• Arginine 100%
• Abzyme 100%
• Enzymatic Hydrolysis 50%
• Escherichia coli 25%
• Wild Type 25%
• Catalytic Efficiency 25%
• Ligand Binding 25%
• Mutant Protein 25%
• Gene Expression System 25%
• Oxyanion Hole 25%
• Single-Chain Variable Fragment 25%

Immunology and Microbiology

• Site Directed Mutagenesis 100%
• Abzyme 100%
• Arginine 100%
• Histidine 100%
• Escherichia coli 33%
• Wild Type 33%
• Gene Expression System 33%
• Catalytic Efficiency 33%
• Ligand Binding 33%
• Single-Chain Variable Fragment 33%