TY - JOUR
T1 - Site-specific sonoporation of human melanoma cells at the cellular level using high lateral-resolution ultrasonic micro-transducer arrays
AU - Thein, Myo
AU - Cheng, An
AU - Khanna, Payal
AU - Zhang, Chunfeng
AU - Park, Eun Joo
AU - Ahmed, Daniel
AU - Goodrich, Christopher J.
AU - Asphahani, Fareid
AU - Wu, Fengbing
AU - Smith, Nadine B.
AU - Dong, Cheng
AU - Jiang, Xiaoning
AU - Zhang, Miqin
AU - Xu, Jian
N1 - Funding Information:
The authors acknowledge the funding support from the National Institutes of Health (NIH/GMS) (Grant No. R01GM075095 ), Chong-Haw Kwang (Bioengineering, PSU) in helping cell culture and fluorescence microscopy, Nicole Zembower (The Huck Institutes of the Life Sciences, PSU) in helping Confocal fluorescence microscopy. Thanks are also due to engineering staff at Penn State Nanofabrication Facility (Materials Research Institute, PSU) for tool trainings and technical assistance in device fabrication. Jian Xu also acknowledges support from National Natural Science Foundation of China, Shanghai Municipal Education Commission and Shanghai Education Development Foundation, and Program for New Century Excellent Talents in University.
PY - 2011/9/15
Y1 - 2011/9/15
N2 - We developed a new instrumental method by which human melanoma cells (LU1205) are sonoporated via radiation pressures exerted by highly-confined ultrasonic waves produced by high lateral-resolution ultrasonic micro-transducer arrays (UMTAs). The method enables cellular-level site-specific sonoporation within the cell monolayer due to UMTAs and can be applicable in the delivery of drugs and gene products in cellular assays. In this method, cells are seeded on the biochip that employs UMTAs for high spatial resolution and specificity. UMTAs are driven by 30-MHz sinusoidal signals and the resulting radiation pressures induce sonoporation in the targeted cells. The sonoporation degree and the effective lateral resolution of UMTAs are determined by performing fluorescent microscopy and analysis of carboxylic-acid-derivatized CdSe/ZnS quantum dots passively transported into the cells. Models representing the transducer-generated ultrasound radiation pressure, the ultrasound-inflicted cell membrane wound, and the transmembrane transport through the wound are developed to determine the ultrasound-pressure-dependent wound size and enhanced cellular uptake of nanoparticles. Model-based calculations show that the effective wound size and cellular uptake of nanoparticles increase linearly with increasing ultrasound pressure (i.e., at applied radiation pressures of 0.21, 0.29, and 0.40. MPa, the ultrasound-induced initial effective wound radii are 150, 460, and 650. nm, respectively, and the post-sonoporation intracellular quantum-dot concentrations are 7.8, 22.8, and 29.9. nM, respectively) and the threshold pressure required to induce sonoporation in LU1205 cells is ~0.12 MPa.
AB - We developed a new instrumental method by which human melanoma cells (LU1205) are sonoporated via radiation pressures exerted by highly-confined ultrasonic waves produced by high lateral-resolution ultrasonic micro-transducer arrays (UMTAs). The method enables cellular-level site-specific sonoporation within the cell monolayer due to UMTAs and can be applicable in the delivery of drugs and gene products in cellular assays. In this method, cells are seeded on the biochip that employs UMTAs for high spatial resolution and specificity. UMTAs are driven by 30-MHz sinusoidal signals and the resulting radiation pressures induce sonoporation in the targeted cells. The sonoporation degree and the effective lateral resolution of UMTAs are determined by performing fluorescent microscopy and analysis of carboxylic-acid-derivatized CdSe/ZnS quantum dots passively transported into the cells. Models representing the transducer-generated ultrasound radiation pressure, the ultrasound-inflicted cell membrane wound, and the transmembrane transport through the wound are developed to determine the ultrasound-pressure-dependent wound size and enhanced cellular uptake of nanoparticles. Model-based calculations show that the effective wound size and cellular uptake of nanoparticles increase linearly with increasing ultrasound pressure (i.e., at applied radiation pressures of 0.21, 0.29, and 0.40. MPa, the ultrasound-induced initial effective wound radii are 150, 460, and 650. nm, respectively, and the post-sonoporation intracellular quantum-dot concentrations are 7.8, 22.8, and 29.9. nM, respectively) and the threshold pressure required to induce sonoporation in LU1205 cells is ~0.12 MPa.
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U2 - 10.1016/j.bios.2011.05.026
DO - 10.1016/j.bios.2011.05.026
M3 - Article
C2 - 21783355
AN - SCOPUS:79960902536
SN - 0956-5663
VL - 27
SP - 25
EP - 33
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
IS - 1
ER -