SLC25A23 augments mitochondrial Ca2+ uptake, interacts with MCU, and induces oxidative stress-mediated cell death

Nicholas E. Hoffman, Harish C. Chandramoorthy, Santhanam Shanmughapriya, Xueqian Q. Zhang, Sandhya Vallem, Patrick J. Doonan, Karthik Malliankaraman, Shuchi Guo, Sudarsan Rajan, John W. Elrod, Walter J. Koch, Joseph Y. Cheung, Muniswamy Madesh

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114 Scopus citations


Emerging findings suggest that two lineages of mitochondrial Ca 2+ uptake par ticipate during active and resting states: 1) the major eukaryotic membrane potential-dependent mitochondrial Ca2+ uniporter and 2) the evolutionarily conserved exchangers and solute carriers, which are also involved in ion transport. Although the influx of Ca2+ across the inner mitochondrial membrane maintains metabolic functions and cell death signal transduction, the mechanisms that regulate mitochondrial Ca2+ accumulation are unclear. Solute carriers - solute carrier 25A23 (SLC25A23), SLC25A24, and SLC25A25 - represent a family of EF-hand-containing mitochondrial proteins that transport Mg-ATP/Pi across the inner membrane. RNA interference-mediated knockdown of SLC25A23 but not SLC25A24 and SLC25A25 decreases mitochondrial Ca2+ uptake and reduces cytosolic Ca 2+ clearance after histamine stimulation. Ectopic expression of SLC25A23 EF-hand-domain mutants exhibits a dominant-negative phenotype of reduced mitochondrial Ca2+ uptake. In addition, SLC25A23 interacts with mito chondrial Ca2+ uniporter (MCU; CCDC109A) and MICU1 (CBARA1) while also increasing IMCU. In addition, SLC25A23 knockdown lowers basal mROS accumulation, attenuates oxidant-induced ATP decline, and reduces cell death. Further, reconstitution with short hairpin RNA-insensitive SLC25A23 cDNA restores mitochondrial Ca2+ uptake and superoxide production. These findings indicate that SLC25A23 plays an important role in mitochondrial matrix Ca2+ influx.

Original languageEnglish (US)
Pages (from-to)936-947
Number of pages12
JournalMolecular biology of the cell
Issue number6
StatePublished - Mar 15 2014

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology


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