Solution structure and dynamics of the bioactive retroviral M domain from Rous sarcoma virus

James M. McDonnell, David Fushman, Sean M. Cahill, Wenjun Zhou, Amy Wolven, Carol B. Wilson, Timothy D. Nelle, Marilyn D. Resh, John Wills, David Cowburn

    Research output: Contribution to journalArticlepeer-review

    47 Scopus citations

    Abstract

    A biologically active construct of the retroviral M domain from the avian Rous sarcoma virus is defined and its solution structure described. This M domain is fully active in budding and infectivity without myristylation. In spite of a sequence homology level that suggests no relationship among M domains and the family of matrix proteins in mammalian retroviruses, the conserved structural elements of a central core allow an M domain sequence motif to be described for all retroviruses. The surface of the M domain has a highly clustered positive patch comprised of sequentially distant residues. An analysis of the backbone dynamics, incorporating rotational anisotropy, is used to estimate the thermodynamics of proposed domain oligomerization.

    Original languageEnglish (US)
    Pages (from-to)921-928
    Number of pages8
    JournalJournal of Molecular Biology
    Volume279
    Issue number4
    DOIs
    StatePublished - Jun 19 1998

    All Science Journal Classification (ASJC) codes

    • Structural Biology
    • Molecular Biology

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