Sonodynamic effects of hematoporphyrin monomethyl ether on Staphylococcus aureus in vitro

Deshu Zhuang; Chunyan Hou; Liangjia Bi; Jialong Han; Yanru Hao; Wenwu Cao; Qi Zhou

doi:10.1111/1574-6968.12628

Sonodynamic effects of hematoporphyrin monomethyl ether on Staphylococcus aureus in vitro

Deshu Zhuang, Chunyan Hou, Liangjia Bi, Jialong Han, Yanru Hao, Wenwu Cao, Qi Zhou

Research output: Contribution to journal › Letter › peer-review

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Abstract

This study aims to investigate the effect of hematoporphyrin monomethyl ether (HMME)-mediated sonodynamic antimicrobial chemotherapy (SACT) on Staphylococcus aureus. SACT was carried out using HMME and 1 MHz ultrasound irradiation. The bactericidal effect was evaluated by the counting colony-forming units (CFU), and important SACT parameters including ultrasound intensity and HMME concentration were determined. More than 95% of the bacteria colonies were effectively killed in the SACT group by 50 μg mL^-1 HMME combined with 6 W cm^-2 tone-burst ultrasound at 1 MHz, but this ultrasound level without HMME only reduced CFU by 38%. In the sonodynamic treatment, higher HMME concentrations and higher ultrasound intensities caused more death of bacteria. Incubation with different HMME concentrations without ultrasound showed no effect. Our results show that the HMME-mediated SACT can be significantly in killing S. aureus.

Original language	English (US)
Pages (from-to)	174-180
Number of pages	7
Journal	FEMS Microbiology Letters
Volume	361
Issue number	2
DOIs	https://doi.org/10.1111/1574-6968.12628
State	Published - Dec 1 2014

All Science Journal Classification (ASJC) codes

Microbiology
Molecular Biology
Genetics

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10.1111/1574-6968.12628

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title = "Sonodynamic effects of hematoporphyrin monomethyl ether on Staphylococcus aureus in vitro",

abstract = "This study aims to investigate the effect of hematoporphyrin monomethyl ether (HMME)-mediated sonodynamic antimicrobial chemotherapy (SACT) on Staphylococcus aureus. SACT was carried out using HMME and 1 MHz ultrasound irradiation. The bactericidal effect was evaluated by the counting colony-forming units (CFU), and important SACT parameters including ultrasound intensity and HMME concentration were determined. More than 95% of the bacteria colonies were effectively killed in the SACT group by 50 μg mL-1 HMME combined with 6 W cm-2 tone-burst ultrasound at 1 MHz, but this ultrasound level without HMME only reduced CFU by 38%. In the sonodynamic treatment, higher HMME concentrations and higher ultrasound intensities caused more death of bacteria. Incubation with different HMME concentrations without ultrasound showed no effect. Our results show that the HMME-mediated SACT can be significantly in killing S. aureus.",

author = "Deshu Zhuang and Chunyan Hou and Liangjia Bi and Jialong Han and Yanru Hao and Wenwu Cao and Qi Zhou",

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T1 - Sonodynamic effects of hematoporphyrin monomethyl ether on Staphylococcus aureus in vitro

AU - Zhuang, Deshu

AU - Hou, Chunyan

AU - Bi, Liangjia

AU - Han, Jialong

AU - Hao, Yanru

AU - Cao, Wenwu

AU - Zhou, Qi

PY - 2014/12/1

Y1 - 2014/12/1

N2 - This study aims to investigate the effect of hematoporphyrin monomethyl ether (HMME)-mediated sonodynamic antimicrobial chemotherapy (SACT) on Staphylococcus aureus. SACT was carried out using HMME and 1 MHz ultrasound irradiation. The bactericidal effect was evaluated by the counting colony-forming units (CFU), and important SACT parameters including ultrasound intensity and HMME concentration were determined. More than 95% of the bacteria colonies were effectively killed in the SACT group by 50 μg mL-1 HMME combined with 6 W cm-2 tone-burst ultrasound at 1 MHz, but this ultrasound level without HMME only reduced CFU by 38%. In the sonodynamic treatment, higher HMME concentrations and higher ultrasound intensities caused more death of bacteria. Incubation with different HMME concentrations without ultrasound showed no effect. Our results show that the HMME-mediated SACT can be significantly in killing S. aureus.

AB - This study aims to investigate the effect of hematoporphyrin monomethyl ether (HMME)-mediated sonodynamic antimicrobial chemotherapy (SACT) on Staphylococcus aureus. SACT was carried out using HMME and 1 MHz ultrasound irradiation. The bactericidal effect was evaluated by the counting colony-forming units (CFU), and important SACT parameters including ultrasound intensity and HMME concentration were determined. More than 95% of the bacteria colonies were effectively killed in the SACT group by 50 μg mL-1 HMME combined with 6 W cm-2 tone-burst ultrasound at 1 MHz, but this ultrasound level without HMME only reduced CFU by 38%. In the sonodynamic treatment, higher HMME concentrations and higher ultrasound intensities caused more death of bacteria. Incubation with different HMME concentrations without ultrasound showed no effect. Our results show that the HMME-mediated SACT can be significantly in killing S. aureus.

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Sonodynamic effects of hematoporphyrin monomethyl ether on Staphylococcus aureus in vitro

Abstract

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