Premise of the study: Genus-specifi c microsatellite markers were developed for Sophora for population genetic and systematic studies of the group in New Zealand, and potentially elsewhere in the geographic range. Methods and Results: From sequencing a totalgenomic DNA library (using Roche 454), we identifi ed and developed 29 polymorphic microsatellite markers for S. microphylla and S. chathamica . We tested 12 of these markers on 14 S. chathamica individuals and four S. microphylla populations. Allloci amplifi ed in both species and species-specifi c alleles occurred at seven loci. In S. microphylla populations, the observed and expected heterozygosities ranged from 0.000-0.960 and 0.000-0.908, respectively, with alleles per locus ranging from seven to 23. Conclusions: The developed markers willbe valuable in studies of phylogenetics, population structure, mating system, and selection of provenances for restoration projects.
All Science Journal Classification (ASJC) codes
- Ecology, Evolution, Behavior and Systematics
- Plant Science