TY - JOUR
T1 - Sophora microphylla (fabaceae) microsatellite markers and their utility across the genus
AU - Van Etten, Megan L.
AU - Houliston, Gary J.
AU - Mitchell, Caroline M.
AU - Heenan, Peter B.
AU - Robertson, Alastair W.
AU - Tate, Jennifer A.
N1 - Publisher Copyright:
© 2014 Smith. Published by the BotanicalSociety of America.
PY - 2014/3
Y1 - 2014/3
N2 - Premise of the study: Genus-specifi c microsatellite markers were developed for Sophora for population genetic and systematic studies of the group in New Zealand, and potentially elsewhere in the geographic range. Methods and Results: From sequencing a totalgenomic DNA library (using Roche 454), we identifi ed and developed 29 polymorphic microsatellite markers for S. microphylla and S. chathamica . We tested 12 of these markers on 14 S. chathamica individuals and four S. microphylla populations. Allloci amplifi ed in both species and species-specifi c alleles occurred at seven loci. In S. microphylla populations, the observed and expected heterozygosities ranged from 0.000-0.960 and 0.000-0.908, respectively, with alleles per locus ranging from seven to 23. Conclusions: The developed markers willbe valuable in studies of phylogenetics, population structure, mating system, and selection of provenances for restoration projects.
AB - Premise of the study: Genus-specifi c microsatellite markers were developed for Sophora for population genetic and systematic studies of the group in New Zealand, and potentially elsewhere in the geographic range. Methods and Results: From sequencing a totalgenomic DNA library (using Roche 454), we identifi ed and developed 29 polymorphic microsatellite markers for S. microphylla and S. chathamica . We tested 12 of these markers on 14 S. chathamica individuals and four S. microphylla populations. Allloci amplifi ed in both species and species-specifi c alleles occurred at seven loci. In S. microphylla populations, the observed and expected heterozygosities ranged from 0.000-0.960 and 0.000-0.908, respectively, with alleles per locus ranging from seven to 23. Conclusions: The developed markers willbe valuable in studies of phylogenetics, population structure, mating system, and selection of provenances for restoration projects.
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U2 - 10.3732/apps.1300081
DO - 10.3732/apps.1300081
M3 - Article
AN - SCOPUS:84943519758
SN - 2168-0450
VL - 2
JO - Applications in Plant Sciences
JF - Applications in Plant Sciences
IS - 3
M1 - 1300081
ER -