@article{703980fbfe324084848f43429384b577,
title = "Spatial control of branching within dendritic arbors by dynein-dependent transport of Rab5-endosomes",
abstract = "Dendrites allow neurons to integrate sensory or synaptic inputs, and the spatial disposition and local density of branches within the dendritic arbor limit the number and type of inputs. Drosophila melanogaster dendritic arborization (da) neurons provide a model system to study the genetic programs underlying such geometry in vivo. Here we report that mutations of motor-protein genes, including a dynein subunit gene (dlic) and kinesin heavy chain (khc), caused not only downsizing of the overall arbor, but also a marked shift of branching activity to the proximal area within the arbor. This phenotype was suppressed when dominant-negative Rab5 was expressed in the mutant neurons, which deposited early endosomes in the cell body. We also showed that 1) in dendritic branches of the wild-type neurons, Rab5-containing early endosomes were dynamically transported and 2) when Rab5 function alone was abrogated, terminal branches were almost totally deleted. These results reveal an important link between microtubule motors and endosomes in dendrite morphogenesis.",
author = "Daisuke Satoh and Daichi Sato and Taiichi Tsuyama and Motoki Saito and Hiroyuki Ohkura and Rolls, {Melissa M.} and Fuyuki Ishikawa and Tadashi Uemura",
note = "Funding Information: The reagents were provided by the Developmental Studies Hybridoma Bank at the University of Iowa, the Bloomington Stock Center, the Drosophila Genetic Resource Center at Kyoto Institute of Technology, J. Knoblich, D. Bilder, L. Luo, Y. N. Jan, H. Shimizu and W. Saxton. We thank Yuh-Nung Jan for communicating data before publication. We are grateful to K. Sugimura, K. Kousaka, T. Kiyomitsu, C. Obuse, M. Sone, Y. Okada, E. Gavis and M. Ohno for their technical advice and encouragement; T. Harumoto and S. Yonehara for allowing us to use their equipment; Y. Miyake and M. Futamata for their technical assistance. This work was supported by grants from the programs Grants-in-Aid for Scientific Research on Priority Areas-Molecular Brain Science (17024025 to T.U.) and for Cancer Research (to F.I. and M.S.) of the MEXT of Japan, by a Wellcome Trust Senior Research Fellowship (to H.O.), and by a NARSAD Young Investigator Award (to M.M.R.). D.S is a recipient of a Fellowship of the Japan Society for the Promotion of Science for Junior Scientists.",
year = "2008",
doi = "10.1038/ncb1776",
language = "English (US)",
volume = "10",
pages = "1164--1171",
journal = "Nature Cell Biology",
issn = "1465-7392",
publisher = "Nature Research",
number = "10",
}