TY - GEN
T1 - Spatial distribution of Burkholderia mallei genome in Punjab, Pakistan
AU - Ali, Muhammad Asad
AU - Muhammad, Khushi
AU - Rabbani, Masood
AU - Anjum, Aftab Ahmad
AU - Ahmad, Mansur Ud Din
AU - Shabbir, Muhammad Zubair
AU - Chaudhry, Muhammad Hamid
AU - Jayarao, Bhushan M.
N1 - Publisher Copyright:
© 2017 IEEE.
PY - 2017/3/1
Y1 - 2017/3/1
N2 - Burkholderia mallei, a zoonotic pathogen, causes glanders in equines. The equids acquire infection from contaminated soil, water and excretions. A study was conducted to determine the geo-spatial distribution of B. mallei genome in soil samples of 10% percent villages (n=456) from eight districts of Punjab province, Pakistan by real time Polymerase chain reaction. Soil samples (n=2,280) from spatially distributed positive localities for B. mallei genome were maped in relation to canals, rivers, roads and railway lines. Real time-PCR was used for detection of B. mallei genome from soil samples wth positive control (118 copies/reaction) of the DNA. Eleven (0.48%) out of 2, 280 soil samples were positive for B. mallei genome distributed in varied locations of Punjab. The samples collected from Sheikhupura district showed higher prevalence (2.37%) followed by Chakwal district (2.10%). None of the samples from Gujranwala, Sahiwal, DG Khan, Attock, Faisalabad and Sargodha districts were found positive for B. mallei genome. The genome of B. mallei was distributed in 25% study districts of Punjab, Pakistan. Findings of the study suggested that RT-PCR assay could be used for detection of B. mallei in clinical samples.
AB - Burkholderia mallei, a zoonotic pathogen, causes glanders in equines. The equids acquire infection from contaminated soil, water and excretions. A study was conducted to determine the geo-spatial distribution of B. mallei genome in soil samples of 10% percent villages (n=456) from eight districts of Punjab province, Pakistan by real time Polymerase chain reaction. Soil samples (n=2,280) from spatially distributed positive localities for B. mallei genome were maped in relation to canals, rivers, roads and railway lines. Real time-PCR was used for detection of B. mallei genome from soil samples wth positive control (118 copies/reaction) of the DNA. Eleven (0.48%) out of 2, 280 soil samples were positive for B. mallei genome distributed in varied locations of Punjab. The samples collected from Sheikhupura district showed higher prevalence (2.37%) followed by Chakwal district (2.10%). None of the samples from Gujranwala, Sahiwal, DG Khan, Attock, Faisalabad and Sargodha districts were found positive for B. mallei genome. The genome of B. mallei was distributed in 25% study districts of Punjab, Pakistan. Findings of the study suggested that RT-PCR assay could be used for detection of B. mallei in clinical samples.
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U2 - 10.1109/IBCAST.2017.7868056
DO - 10.1109/IBCAST.2017.7868056
M3 - Conference contribution
AN - SCOPUS:85029801507
T3 - Proceedings of 2017 14th International Bhurban Conference on Applied Sciences and Technology, IBCAST 2017
SP - 197
EP - 213
BT - Proceedings of 2017 14th International Bhurban Conference on Applied Sciences and Technology, IBCAST 2017
A2 - Zafar-uz-Zaman, Muhammad
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 14th International Bhurban Conference on Applied Sciences and Technology, IBCAST 2017
Y2 - 10 January 2017 through 14 January 2017
ER -