TY - JOUR
T1 - Spatiotemporal heterogeneity of CNS radial glial cells and their transition to restricted precursors
AU - Li, Hedong
AU - Babiarz, Joanne
AU - Woodbury, Jennifer
AU - Kane-Goldsmith, Noriko
AU - Grumet, Martin
N1 - Funding Information:
We thank Drs. Nat Heintz, Randall McKinnon, Mahendra Rao, Thomas Jessell, Zaven Kaprielian and Joel Levine for antibodies, and Dr. Evan Snyder for retrovirus. We are grateful to Drs. Randall McKinnon, Zaven Kaprielian, Michael Matise and Nick Gaiano for helpful discussion and comments on the manuscript. This study was supported by NIH Grant NS338112 (MG) and New Jersey Commission on Spinal Cord Research NJCSCR 007-101 (HL).
PY - 2004/7/15
Y1 - 2004/7/15
N2 - Radial glia are among the first cells that develop in the embryonic central nervous system. They are progenitors of glia and neurons but their relationship with restricted precursors that are also derived from neuroepithelia is unclear. To clarify this issue, we analyzed expression of cell type specific markers (BLBP for radial glia, 5A5/E-NCAM for neuronal precursors and A2B5 for glial precursors) on cortical radial glia in vivo and their progeny in vitro. Clones of cortical cells initially expressing only BLBP gave rise to cells that were A2B5+ and eventually lost BLBP expression in vitro. BLBP is expressed in the rat neuroepithelium as early as E12.5 when there is little or no staining for A2B5 and 5A5. In E13.5-15.5 forebrain, A2B5 is spatially restricted co-localizing with a subset of the BLBP+ radial glia. Analysis of cells isolated acutely from embryonic cortices confirmed that BLBP expression could appear without, or together with, A2B5 or 5A5. The numbers of BLBP+/5A5+ cells decreased during neurogenesis while the numbers of BLBP+/A2B5+ cells remained high through the beginning of gliogenesis. The combined results demonstrate that spatially restricted subpopulations of radial glia along the dorsal-ventral axis acquire different markers for neuronal or glial precursors during CNS development.
AB - Radial glia are among the first cells that develop in the embryonic central nervous system. They are progenitors of glia and neurons but their relationship with restricted precursors that are also derived from neuroepithelia is unclear. To clarify this issue, we analyzed expression of cell type specific markers (BLBP for radial glia, 5A5/E-NCAM for neuronal precursors and A2B5 for glial precursors) on cortical radial glia in vivo and their progeny in vitro. Clones of cortical cells initially expressing only BLBP gave rise to cells that were A2B5+ and eventually lost BLBP expression in vitro. BLBP is expressed in the rat neuroepithelium as early as E12.5 when there is little or no staining for A2B5 and 5A5. In E13.5-15.5 forebrain, A2B5 is spatially restricted co-localizing with a subset of the BLBP+ radial glia. Analysis of cells isolated acutely from embryonic cortices confirmed that BLBP expression could appear without, or together with, A2B5 or 5A5. The numbers of BLBP+/5A5+ cells decreased during neurogenesis while the numbers of BLBP+/A2B5+ cells remained high through the beginning of gliogenesis. The combined results demonstrate that spatially restricted subpopulations of radial glia along the dorsal-ventral axis acquire different markers for neuronal or glial precursors during CNS development.
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U2 - 10.1016/j.ydbio.2004.02.028
DO - 10.1016/j.ydbio.2004.02.028
M3 - Article
C2 - 15223331
AN - SCOPUS:3042680192
SN - 0012-1606
VL - 271
SP - 225
EP - 238
JO - Developmental biology
JF - Developmental biology
IS - 2
ER -