Spezifische serologische untersuchungen mit einem neuartigen authentischen HPV-antigen (Virus-like particles) auf HPV-6 antikorper bei gynakologischen patientenkollektiven

Objective: A serological assay lkw genital IIPV infection whould provide important additional information to HPV DNA diagnostic methods, since it would evaluate prior exposure to the viruses, detect significant systemic immunologic response to virus infection, and could he performed in most clinical laboratories. Methods: Serum samples from three groups of patients attending a gynecology clinic were analysed by direct ELISA for specific IgG-antibodies to baculovirus-expressed HPV-6-and BPV-1-L1-VLPs. Results: Positive IgG reactivity to HPV-6-L1-QIN were 4172 (6%) in a control group, 28/73 (38%) in a condyloma group and 17/62 (17%) in cervical intraepithelial neoplasia patients. Individual EgG ELISA values of condyloma and ON patients for HPV-6-L1-VLPs demonstrated no correlation to results with BPV-I-LI-VLPs. Conclusions: These data show that HPV-6-L1-VLPs are effective antigens for serological studies and can detect species specific antibodies with important implications for diagnosis. epidemiology. insites to natural course of disease. prognosis and evaluation of vaccination.