TY - JOUR
T1 - Spontaneously arising red cells with a McLeod-like phenotype in normal donors
AU - Araten, David J.
AU - Sanders, Katie J.
AU - Pu, Jeffrey
AU - Lee, Soohee
N1 - Funding Information:
Supported in part by NIH grants RO1 CA-109258 (DJA) and RO1 HL075716 (SL). We are indebted to Dr. Marion Reid of the New York Blood Center for assistance in obtaining control red cell samples, Gregory Halverson of the New York Blood Center for assistance in obtaining K14 hybridoma supernatant, and to Bridget Lane of the NYU Cancer Center for assistance in coordinating the collection of blood samples from healthy donors. We would like to thank Dr. Ed Luther for assistance with the iCyte analysis.
PY - 2009/12/1
Y1 - 2009/12/1
N2 - Very few human genes can be used to identify spontaneous inactivating somatic mutations. We hypothesized that because the XK gene is X-linked, it would be easy to identify spontaneously arising red cells with a phenotype resembling the McLeod syndrome, which results from inherited XK mutations. Here, by flow cytometry, we detect such phenotypic variants at a median frequency of 9 × 10-6 in neonatal cord blood samples and 39 × 10-6 in healthy adults (p = 0.004). It may be possible to further investigate the relationship between aging, mutations, and cancer using this approach.
AB - Very few human genes can be used to identify spontaneous inactivating somatic mutations. We hypothesized that because the XK gene is X-linked, it would be easy to identify spontaneously arising red cells with a phenotype resembling the McLeod syndrome, which results from inherited XK mutations. Here, by flow cytometry, we detect such phenotypic variants at a median frequency of 9 × 10-6 in neonatal cord blood samples and 39 × 10-6 in healthy adults (p = 0.004). It may be possible to further investigate the relationship between aging, mutations, and cancer using this approach.
UR - https://www.scopus.com/pages/publications/70350298971
UR - https://www.scopus.com/pages/publications/70350298971#tab=citedBy
U2 - 10.1016/j.mrfmmm.2009.03.009
DO - 10.1016/j.mrfmmm.2009.03.009
M3 - Article
C2 - 19909712
AN - SCOPUS:70350298971
SN - 0027-5107
VL - 671
SP - 1
EP - 5
JO - Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
JF - Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
IS - 1-2
ER -
