TY - JOUR
T1 - SRV2, a gene required for RAS activation of adenylate cyclase in yeast
AU - Fedor-Chaiken, Mary
AU - Deschenes, Robert J.
AU - Broach, James R.
N1 - Funding Information:
We are indebted to Gan-Ju Shei for her excellent technical assistance. We would also like to thank Scott Powers for construction of the URA3 insertion at the Pstl site of SRM, Ken Ferguson for assistance with the CAMP assay, and Jeff Fields and Michael Wigler for sharing results prior to submission for publication. This work was supported by grant CA41086 from the National Cancer Institute of the National Institutes of Health.
PY - 1990/4/20
Y1 - 1990/4/20
N2 - We have identified a gene, SRV2, mutations of which alleviate stress sensitivity in strains carrying an activated RAS gene. Epistasis analysis suggests that the gene affects accumulation of cAMP in the cell. Direct assays of cAMP accumulation indicate that mutations of the gene diminish the rate of in vivo production of cAMP following stimulation by an activated RAS allele. Null mutations of srv2 result in lethality, which cannot be suppressed by mutational activation of the cAMP-dependent protein kinase. The sequence of the gene indicates that it encodes an adenylate cyclase-associated protein. These results demonstrate that SRV2 protein is required for RAS-activated adenylate cyclase activity, but that it participates in other essential cellular functions as well.
AB - We have identified a gene, SRV2, mutations of which alleviate stress sensitivity in strains carrying an activated RAS gene. Epistasis analysis suggests that the gene affects accumulation of cAMP in the cell. Direct assays of cAMP accumulation indicate that mutations of the gene diminish the rate of in vivo production of cAMP following stimulation by an activated RAS allele. Null mutations of srv2 result in lethality, which cannot be suppressed by mutational activation of the cAMP-dependent protein kinase. The sequence of the gene indicates that it encodes an adenylate cyclase-associated protein. These results demonstrate that SRV2 protein is required for RAS-activated adenylate cyclase activity, but that it participates in other essential cellular functions as well.
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U2 - 10.1016/0092-8674(90)90813-T
DO - 10.1016/0092-8674(90)90813-T
M3 - Article
C2 - 2158860
AN - SCOPUS:0025361043
SN - 0092-8674
VL - 61
SP - 329
EP - 340
JO - Cell
JF - Cell
IS - 2
ER -