SSCP analysis of scnDNA for genetic profiling of Aedes aegypti

Jacklyn Wong; Frédéric Tripet; Jason L. Rasgon; Gregory C. Lanzaro; Thomas W. Scott

doi:10.4269/ajtmh.2008.79.511

SSCP analysis of scnDNA for genetic profiling of Aedes aegypti

Jacklyn Wong, Frédéric Tripet, Jason L. Rasgon, Gregory C. Lanzaro, Thomas W. Scott

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

We characterized genetic profiling markers for Aedes aegypti using single-strand conformation polymorphism (SSCP) analysis of single copy nuclear genes (scnDNA). Nucleotide variations at 18 loci were evaluated in 173 wild Ae. aegypti collected from a single population in northwestern Thailand. We identified seven scnDNAs with polymorphisms sufficient to determine a unique genetic profile for each mosquito examined. Six markers were derived from previously mapped cDNA loci. One marker was developed from a non-coding region of a gene. The number of alleles at each scnDNA locus ranged from 3 to 9. The described scnDNAs can be used to quickly fingerprint large numbers of Ae. aegypti to track the behavior of individual mosquitoes in the field.

Original language	English (US)
Pages (from-to)	511-517
Number of pages	7
Journal	American Journal of Tropical Medicine and Hygiene
Volume	79
Issue number	4
DOIs	https://doi.org/10.4269/ajtmh.2008.79.511
State	Published - Oct 2008

All Science Journal Classification (ASJC) codes

Parasitology
Virology
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.4269/ajtmh.2008.79.511

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title = "SSCP analysis of scnDNA for genetic profiling of Aedes aegypti",

abstract = "We characterized genetic profiling markers for Aedes aegypti using single-strand conformation polymorphism (SSCP) analysis of single copy nuclear genes (scnDNA). Nucleotide variations at 18 loci were evaluated in 173 wild Ae. aegypti collected from a single population in northwestern Thailand. We identified seven scnDNAs with polymorphisms sufficient to determine a unique genetic profile for each mosquito examined. Six markers were derived from previously mapped cDNA loci. One marker was developed from a non-coding region of a gene. The number of alleles at each scnDNA locus ranged from 3 to 9. The described scnDNAs can be used to quickly fingerprint large numbers of Ae. aegypti to track the behavior of individual mosquitoes in the field.",

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AU - Scott, Thomas W.

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SSCP analysis of scnDNA for genetic profiling of Aedes aegypti

Abstract

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