Abstract
We characterized genetic profiling markers for Aedes aegypti using single-strand conformation polymorphism (SSCP) analysis of single copy nuclear genes (scnDNA). Nucleotide variations at 18 loci were evaluated in 173 wild Ae. aegypti collected from a single population in northwestern Thailand. We identified seven scnDNAs with polymorphisms sufficient to determine a unique genetic profile for each mosquito examined. Six markers were derived from previously mapped cDNA loci. One marker was developed from a non-coding region of a gene. The number of alleles at each scnDNA locus ranged from 3 to 9. The described scnDNAs can be used to quickly fingerprint large numbers of Ae. aegypti to track the behavior of individual mosquitoes in the field.
Original language | English (US) |
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Pages (from-to) | 511-517 |
Number of pages | 7 |
Journal | American Journal of Tropical Medicine and Hygiene |
Volume | 79 |
Issue number | 4 |
DOIs | |
State | Published - Oct 2008 |
All Science Journal Classification (ASJC) codes
- Parasitology
- Virology
- Infectious Diseases