Stability of opioid growth factor ([Met5]-enkephalin) in solution using HPLC and photodiode array detection

W. Jeffrey Hurst; Patricia J. McLaughlin; Ian S. Zagon; Moshe Rogosnitzky

doi:10.1080/10826070500416395

Stability of opioid growth factor ([Met⁵]-enkephalin) in solution using HPLC and photodiode array detection

W. Jeffrey Hurst
, Patricia J. McLaughlin
, Ian S. Zagon
, Moshe Rogosnitzky

Research output: Contribution to journal › Article › peer-review

Abstract

A reversed phased HPLC method with photodiode array detection was used to monitor the stability of opioid growth factor (OGF; [Met⁵]- enkephalin) in sterile saline for 1, 2, and 8 weeks, and stored at -17°C, 4°C, and 23°C. Samples maintained at -17°C had 97% of the initial OGF concentration after 2 months, whereas those stored at 4°C had 94% of the original concentration. Preparations housed at 22°C had reductions of 6-12% by the 8th week. These data provide important information about storage of OGF for clinical applications.

Original language	English (US)
Pages (from-to)	151-157
Number of pages	7
Journal	Journal of Liquid Chromatography and Related Technologies
Volume	29
Issue number	2
DOIs	https://doi.org/10.1080/10826070500416395
State	Published - Jan 1 2006

All Science Journal Classification (ASJC) codes

Analytical Chemistry
Biochemistry
Pharmaceutical Science
Clinical Biochemistry

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10.1080/10826070500416395

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title = "Stability of opioid growth factor ([Met5]-enkephalin) in solution using HPLC and photodiode array detection",

abstract = "A reversed phased HPLC method with photodiode array detection was used to monitor the stability of opioid growth factor (OGF; [Met5]- enkephalin) in sterile saline for 1, 2, and 8 weeks, and stored at -17°C, 4°C, and 23°C. Samples maintained at -17°C had 97\% of the initial OGF concentration after 2 months, whereas those stored at 4°C had 94\% of the original concentration. Preparations housed at 22°C had reductions of 6-12\% by the 8th week. These data provide important information about storage of OGF for clinical applications.",

author = "Hurst, \{W. Jeffrey\} and McLaughlin, \{Patricia J.\} and Zagon, \{Ian S.\} and Moshe Rogosnitzky",

note = "Funding Information: This work was supported in part by Philip Morris USA Inc. and Philip Morris International. We thank Shimadzu Scientific Instruments for the loan of the HPLC system.",

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AU - Hurst, W. Jeffrey

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AU - Rogosnitzky, Moshe

N1 - Funding Information: This work was supported in part by Philip Morris USA Inc. and Philip Morris International. We thank Shimadzu Scientific Instruments for the loan of the HPLC system.

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N2 - A reversed phased HPLC method with photodiode array detection was used to monitor the stability of opioid growth factor (OGF; [Met5]- enkephalin) in sterile saline for 1, 2, and 8 weeks, and stored at -17°C, 4°C, and 23°C. Samples maintained at -17°C had 97% of the initial OGF concentration after 2 months, whereas those stored at 4°C had 94% of the original concentration. Preparations housed at 22°C had reductions of 6-12% by the 8th week. These data provide important information about storage of OGF for clinical applications.

AB - A reversed phased HPLC method with photodiode array detection was used to monitor the stability of opioid growth factor (OGF; [Met5]- enkephalin) in sterile saline for 1, 2, and 8 weeks, and stored at -17°C, 4°C, and 23°C. Samples maintained at -17°C had 97% of the initial OGF concentration after 2 months, whereas those stored at 4°C had 94% of the original concentration. Preparations housed at 22°C had reductions of 6-12% by the 8th week. These data provide important information about storage of OGF for clinical applications.

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Stability of opioid growth factor ([Met⁵]-enkephalin) in solution using HPLC and photodiode array detection

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